Antibody Name:	Anti-Cyclin C Antibody
Antibody SKU:	CAB6545
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-283 of human Cyclin C (NP_005181.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, NIH/3T3, Mouse liver, Mouse kidney, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-283 of human Cyclin C (NP_005181.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAGN FWQS SHYL QWIL DKQD LLKE RQKD LKFL SEEE YWKL QIFF TNVI QALG EHLK LRQQ VIAT ATVY FKRF YARY SLKS IDPV LMAP TCVF LASK VEEF GVVS NTRL IAAA TSVL KTRF SYAF PKEF PYRM NHIL ECEF YLLE LMDC CLIV YHPY RPLL QYVQ DMGQ EDML LPLA WRIV NDTY RTDL CLLY PPFM IALA CLHV ACVV QQKD ARQW FAEL SVDM EKIL EIIR VILK LYEQ WKNF DERK EMAT ILSK MPKP KPPP NSEG EQGP NGSQ NSSY SQS
Gene ID:	892
Uniprot:	P24863
Cellular Location:	Nucleus
Calculated MW:	22kDa/33kDa
Observed MW:	26-33kDa

Synonyms:

CCNC, CycC, SRB11, hSRB11, cyclin-C

Background:

The protein encoded by this gene is a member of the cyclin family of proteins. The encoded protein interacts with cyclin-dependent kinase 8 and induces the phophorylation of the carboxy-terminal domain of the large subunit of RNA polymerase II. The level of mRNAs for this gene peaks in the G1 phase of the cell cycle. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	CCNC: Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Binds to and activates cyclin-dependent kinase CDK8 that phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Belongs to the cyclin family. Cyclin C subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cell cycle regulation Chromosomal Location of Human Ortholog: 6q21 Cellular Component: cyclin-dependent protein kinase holoenzyme complex; nucleoplasm; nucleus Molecular Function:cyclin-dependent protein kinase regulator activity; protein binding; protein serine/threonine kinase activity Biological Process: positive regulation of cyclin-dependent protein kinase activity; positive regulation of transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter
NCBI Summary:	The protein encoded by this gene is a member of the cyclin family of proteins. The encoded protein interacts with cyclin-dependent kinase 8 and induces the phophorylation of the carboxy-terminal domain of the large subunit of RNA polymerase II. The level of mRNAs for this gene peaks in the G1 phase of the cell cycle. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	P24863
NCBI GenInfo Identifier:	166214910
NCBI Gene ID:	892
NCBI Accession:	P24863.2
UniProt Secondary Accession:	P24863,Q9H543, B4DPZ1,
UniProt Related Accession:	P24863
Molecular Weight:	22,945 Da
NCBI Full Name:	Cyclin-C
NCBI Synonym Full Names:	cyclin C
NCBI Official Symbol:	CCNC
NCBI Official Synonym Symbols:	CycC
NCBI Protein Information:	cyclin-C
UniProt Protein Name:	Cyclin-C
UniProt Synonym Protein Names:	SRB11 homolog; hSRB11
UniProt Gene Name:	CCNC
UniProt Entry Name:	CCNC_HUMAN

	Western blot analysis of extracts of various cell lines, using Cyclin C antibody (CAB6545) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded rat brain using Cyclin C antibody (CAB6545) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using Cyclin C antibody (CAB6545) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using Cyclin C antibody (CAB6545) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of MCF7 cells using Cyclin C antibody (CAB6545). Blue: DAPI for nuclear staining.