Name: Anti-Cyclin B2 Antibody (CAB3351)
Brand: Cell Cycle Antibodies 1
SKU: CAB3351
Availability: OutOfStock

Antibody Name:	Anti-Cyclin B2 Antibody
Antibody SKU:	CAB3351
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human Cyclin B2 (NP_004692.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, SW480, DU145, A-431, K-562, Mouse thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human Cyclin B2 (NP_004692.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MALL RRPT VSSD LENI DTGV NSKV KSHV TIRR TVLE EIGN RVTT RAAQ VAKK AQNT KVPV QPTK TTNV NKQL KPTA SVKP VQME KLAP KGPS PTPE DVSM
Gene ID:	9133
Uniprot:	O95067
Cellular Location:
Calculated MW:	45kDa
Observed MW:	45kDa

Synonyms:

CCNB2, HsT17299, cyclin B2

Background:

Cyclin B2 is a member of the cyclin family, specifically the B-type cyclins. The B-type cyclins, B1 and B2, associate with p34cdc2 and are essential components of the cell cycle regulatory machinery. B1 and B2 differ in their subcellular localization. Cyclin B1 co-localizes with microtubules, whereas cyclin B2 is primarily associated with the Golgi region. Cyclin B2 also binds to transforming growth factor beta RII and thus cyclin B2/cdc2 may play a key role in transforming growth factor beta-mediated cell cycle control.

UniProt Protein Function:	CCNB2: Essential for the control of the cell cycle at the G2/M (mitosis) transition. Belongs to the cyclin family. Cyclin AB subfamily.
UniProt Protein Details:	Protein type:Cell cycle regulation Chromosomal Location of Human Ortholog: 15q22.2 Cellular Component: microtubule cytoskeleton; nucleoplasm; centrosome; membrane; cytosol Molecular Function:protein binding; protein kinase binding Biological Process: mitosis; thymus development; cell division; regulation of cell cycle; in utero embryonic development; mitotic nuclear envelope disassembly; T cell homeostasis; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; G2/M transition of mitotic cell cycle; growth
NCBI Summary:	Cyclin B2 is a member of the cyclin family, specifically the B-type cyclins. The B-type cyclins, B1 and B2, associate with p34cdc2 and are essential components of the cell cycle regulatory machinery. B1 and B2 differ in their subcellular localization. Cyclin B1 co-localizes with microtubules, whereas cyclin B2 is primarily associated with the Golgi region. Cyclin B2 also binds to transforming growth factor beta RII and thus cyclin B2/cdc2 may play a key role in transforming growth factor beta-mediated cell cycle control. [provided by RefSeq, Jul 2008]
UniProt Code:	O95067
NCBI GenInfo Identifier:	5921731
NCBI Gene ID:	9133
NCBI Accession:	O95067.1
UniProt Related Accession:	O95067
Molecular Weight:	~ 45kDa
NCBI Full Name:	G2/mitotic-specific cyclin-B2
NCBI Synonym Full Names:	cyclin B2
NCBI Official Symbol:	CCNB2
NCBI Official Synonym Symbols:	HsT17299
NCBI Protein Information:	G2/mitotic-specific cyclin-B2
UniProt Protein Name:	G2/mitotic-specific cyclin-B2
Protein Family:	Light-harvesting protein
UniProt Gene Name:	CCNB2
UniProt Entry Name:	CCNB2_HUMAN

Western blot analysis of extracts of various cell lines, using Cyclin B2 antibody (CAB3351) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Antibodies

Anti-Cyclin B2 Antibody (CAB16463)

CCNB2 Antibody (PACO08245)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)