Cell Cycle Antibodies 1

Anti-Cyclin B2 Antibody (CAB16463)

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SKU:
CAB16463
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-Cyclin B2 Antibody
Antibody SKU:CAB16463
Antibody Size:20uL, 50uL, 100uL
Application:WB IP
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human Cyclin B2 (NP_004692.1).
Application:WB IP
Recommended Dilution:WB 1:500 - 1:2000 IP 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:HeLa, SW480, A-431
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human Cyclin B2 (NP_004692.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MALL RRPT VSSD LENI DTGV NSKV KSHV TIRR TVLE EIGN RVTT RAAQ VAKK AQNT KVPV QPTK TTNV NKQL KPTA SVKP VQME KLAP KGPS PTPE DVSM
Gene ID:9133
Uniprot:O95067
Cellular Location:
Calculated MW:45kDa
Observed MW:45kDa
Synonyms:CCNB2, HsT17299, cyclin B2
Background:Cyclin B2 is a member of the cyclin family, specifically the B-type cyclins. The B-type cyclins, B1 and B2, associate with p34cdc2 and are essential components of the cell cycle regulatory machinery. B1 and B2 differ in their subcellular localization. Cyclin B1 co-localizes with microtubules, whereas cyclin B2 is primarily associated with the Golgi region. Cyclin B2 also binds to transforming growth factor beta RII and thus cyclin B2/cdc2 may play a key role in transforming growth factor beta-mediated cell cycle control.
UniProt Protein Function:CCNB2: Essential for the control of the cell cycle at the G2/M (mitosis) transition. Belongs to the cyclin family. Cyclin AB subfamily.
UniProt Protein Details:

Protein type:Cell cycle regulation

Chromosomal Location of Human Ortholog: 15q22.2

Cellular Component: microtubule cytoskeleton; nucleoplasm; centrosome; membrane; cytosol

Molecular Function:protein binding; protein kinase binding

Biological Process: mitosis; thymus development; cell division; regulation of cell cycle; in utero embryonic development; mitotic nuclear envelope disassembly; T cell homeostasis; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; G2/M transition of mitotic cell cycle; growth

NCBI Summary:Cyclin B2 is a member of the cyclin family, specifically the B-type cyclins. The B-type cyclins, B1 and B2, associate with p34cdc2 and are essential components of the cell cycle regulatory machinery. B1 and B2 differ in their subcellular localization. Cyclin B1 co-localizes with microtubules, whereas cyclin B2 is primarily associated with the Golgi region. Cyclin B2 also binds to transforming growth factor beta RII and thus cyclin B2/cdc2 may play a key role in transforming growth factor beta-mediated cell cycle control. [provided by RefSeq, Jul 2008]
UniProt Code:O95067
NCBI GenInfo Identifier:5921731
NCBI Gene ID:9133
NCBI Accession:O95067.1
UniProt Related Accession:O95067
Molecular Weight:~ 45kDa
NCBI Full Name:G2/mitotic-specific cyclin-B2
NCBI Synonym Full Names:cyclin B2
NCBI Official Symbol:CCNB2  
NCBI Official Synonym Symbols:HsT17299  
NCBI Protein Information:G2/mitotic-specific cyclin-B2
UniProt Protein Name:G2/mitotic-specific cyclin-B2
Protein Family:Light-harvesting protein
UniProt Gene Name:CCNB2  
UniProt Entry Name:CCNB2_HUMAN
Anti-Cyclin B2 Antibody (CAB16463)Western blot analysis of extracts of various cell lines, using Cyclin B2 Rabbit pAb (CAB16463) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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