Antibody Name:	Anti-Cyclin A1 Antibody
Antibody SKU:	CAB5631
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human CCNA1 (NP_001104515.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Rat
Positive Samples:	HL-60, U-87MG, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human CCNA1 (NP_001104515.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	METG FPAI MYPG SFIG GWGE EYLS WEGP GLPD FVFQ QPVE SEAM HCSN PKSG VVLA TVAR GPDA CQIL TRAP LGQD PPQR TVLG LLTA NGQY RRTC GQGI TRIR CYSG SENA FPPA GKKA LPDC GVQE PPKQ GFDI YMDE LEQG DRDS CSVR EGMA FEDV YEVD TGTL KSDL HFLL DFNT VSPM LVDS SLLS QSED ISSL GTDV INVT EYAE EIYQ YLRE AEIR HRPK AH
Gene ID:	8900
Uniprot:	P78396
Cellular Location:	Nucleus
Calculated MW:	47kDa/52kDa
Observed MW:	52kDa

Synonyms:

CCNA1, CT146, cyclin-A1

Background:

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. The cyclin encoded by this gene was shown to be expressed in testis and brain, as well as in several leukemic cell lines, and is thought to primarily function in the control of the germline meiotic cell cycle. This cyclin binds both CDK2 and CDC2 kinases, which give two distinct kinase activities, one appearing in S phase, the other in G2, and thus regulate separate functions in cell cycle. This cyclin was found to bind to important cell cycle regulators, such as Rb family proteins, transcription factor E2F-1, and the p21 family proteins. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	CCNA1: May be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. May primarily function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells. Belongs to the cyclin family. Cyclin AB subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cancer Testis Antigen (CTA); Cell cycle regulation; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 13q12.3-q13 Cellular Component: nucleoplasm; microtubule cytoskeleton; cytosol Molecular Function:protein binding; protein kinase binding Biological Process: G1/S-specific transcription in mitotic cell cycle; mitosis; male meiosis I; cell division; regulation of G2/M transition of mitotic cell cycle; spermatogenesis; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; G2/M transition of mitotic cell cycle; G1/S transition of mitotic cell cycle
NCBI Summary:	The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. The cyclin encoded by this gene was shown to be expressed in testis and brain, as well as in several leukemic cell lines, and is thought to primarily function in the control of the germline meiotic cell cycle. This cyclin binds both CDK2 and CDC2 kinases, which give two distinct kinase activities, one appearing in S phase, the other in G2, and thus regulate separate functions in cell cycle. This cyclin was found to bind to important cell cycle regulators, such as Rb family proteins, transcription factor E2F-1, and the p21 family proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	P78396
NCBI GenInfo Identifier:	8134359
NCBI Gene ID:	8900
NCBI Accession:	P78396.1
UniProt Secondary Accession:	P78396,Q5T3V0, Q5U0G2, Q8IY91, B7Z7E3,
UniProt Related Accession:	P78396
Molecular Weight:	465
NCBI Full Name:	Cyclin-A1
NCBI Synonym Full Names:	cyclin A1
NCBI Official Symbol:	CCNA1
NCBI Official Synonym Symbols:	CT146
NCBI Protein Information:	cyclin-A1
UniProt Protein Name:	Cyclin-A1
Protein Family:	Cyclin
UniProt Gene Name:	CCNA1
UniProt Entry Name:	CCNA1_HUMAN

	Western blot analysis of extracts of Rat testis, using Cyclin A1 antibody (CAB5631) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.
	Western blot analysis of extracts of various cell lines, using Cyclin A1 antibody (CAB5631) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.