Description
| Antibody Name: | Anti-CYB5R3 Antibody (CAB20892) |
| Antibody SKU: | CAB20892 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of Human CYB5R3. |
| Application: | Western blotting |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Rat |
| Positive Samples: | HepG2, A-431, K-562, Rat liver, Rat testis |
| Immunogen: | Recombinant protein of Human CYB5R3. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Cellular Location: | Cytoplasm, Cytoplasmic side, Endoplasmic reticulum membrane, Lipid-anchor, Mitochondrion outer membrane |
| Calculated MW: | 31kDa/34kDa/38kDa |
| Observed MW: | 35KDa |
| Synonyms: | CYB5R3, B5R, DIA1 |
| Background: | This gene encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. Alternate splicing results in multiple transcript variants. Mutations in this gene cause methemoglobinemias. |
 | Western blot analysis of extracts of various cell lines, using CYB5R3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. |
