Cell Biology Antibodies 6
Anti-CYB5B Antibody (CAB15900)
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- SKU:
- CAB15900
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-CYB5B Antibody |
| Antibody SKU: | CAB15900 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 16-150 of human CYB5B (NP_085056.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | Raji, HeLa, LO2, Mouse thymus, Mouse brain, Mouse heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 16-150 of human CYB5B (NP_085056.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | KGQE VETS VTYY RLEE VAKR NSLK ELWL VIHG RVYD VTRF LNEH PGGE EVLL EQAG VDAS ESFE DVGH SSDA REML KQYY IGDI HPSD LKPE SGSK DPSK NDTC KSCW AYWI LPII GAVL LGFL YRYY TSES KSS |
| Gene ID: | 80777 |
| Uniprot: | O43169 |
| Cellular Location: | Mitochondrion outer membrane |
| Calculated MW: | 16kDa |
| Observed MW: | 20kDa |
| Synonyms: | CYB5B, CYB5-M, CYPB5M, OMB5 |
| Background: |
| UniProt Protein Function: | CYB5B: Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. Belongs to the cytochrome b5 family. |
| UniProt Protein Details: | Protein type:Oxidoreductase; Mitochondrial; Membrane protein, integral Chromosomal Location of Human Ortholog: 16q22.1 Cellular Component: mitochondrial outer membrane; membrane; integral to membrane Molecular Function:metal ion binding; heme binding |
| UniProt Code: | O43169 |
| NCBI GenInfo Identifier: | 83921614 |
| NCBI Gene ID: | 80777 |
| NCBI Accession: | NP_085056.2 |
| UniProt Secondary Accession: | O43169,Q96CC3, Q9BT35, A8K6B1, |
| UniProt Related Accession: | O43169 |
| Molecular Weight: | 16,332 Da |
| NCBI Full Name: | cytochrome b5 type B |
| NCBI Synonym Full Names: | cytochrome b5 type B (outer mitochondrial membrane) |
| NCBI Official Symbol: | CYB5B |
| NCBI Official Synonym Symbols: | OMB5; CYB5-M; CYPB5M |
| NCBI Protein Information: | cytochrome b5 type B; type 2 cyt-b5; outer mitochondrial membrane cytochrome b5; cytochrome b5 outer mitochondrial membrane isoform |
| UniProt Protein Name: | Cytochrome b5 type B |
| UniProt Synonym Protein Names: | Cytochrome b5 outer mitochondrial membrane isoform |
| Protein Family: | Cytochrome |
| UniProt Gene Name: | CYB5B |
| UniProt Entry Name: | CYB5B_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CYB5B antibody (CAB15900) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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