Name: Anti-CUX1 Antibody (CAB2213)
Brand: Cell Biology Antibodies 8
SKU: CAB2213
Availability: OutOfStock

Antibody Name:	Anti-CUX1 Antibody
Antibody SKU:	CAB2213
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human CUX1 (NP_001189472.1).

Application:	WB IF
Recommended Dilution:	WB 1:1000 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, Jurkat, MCF7, mouse brain, mouse testis, mouse kidney, rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human CUX1 (NP_001189472.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAN VGSM FQYW KRFD LQQL QREL DATA TVLA NRQD ESEQ SRKR LIEQ SREF KKNT PEDL RKQV APLL KSFQ GEID ALSK RSKE AEAA FLNV YKRL IDVP DPVP ALDL GQQL QLKV QRLH DIET ENQK LRET LEEY NKEF AEVK NQEV TIKA LKEK IREY EQTL KNQA ETIA LEKE QKLQ NDFA EKER KLQE TQMS TTSK LEEA EHKV QSLQ TALE KTRT ELFD LKTK YDEE TTAK ADEI EMIM TDLE RANQ RAEV AQRE AETL REQL SSAN HSLQ LASQ
Gene ID:	1523
Uniprot:	P39880/Q13948
Cellular Location:	Golgi apparatus membrane, Single-pass type IV membrane protein
Calculated MW:	72-77kDa/147-165kDa
Observed MW:	90kDa/110kDa/250kDa

Synonyms:	CUX1, CASP, CDP, CDP/Cut, CDP1, COY1, CUTL1, CUX, Clox, Cux/CDP, GOLIM6, Nbla10317, p100, p110, p200, p75
Background:	The protein encoded by this gene is a member of the homeodomain family of DNA binding proteins. It may regulate gene expression, morphogenesis, and differentiation and it may also play a role in the cell cycle progession. Several alternatively spliced transcript variants encoding different isoforms have been identified.

UniProt Protein Function:	CUTL1: Probably has a broad role in mammalian development as a repressor of developmentally regulated gene expression. May act by preventing binding of positively-activing CCAAT factors to promoters. Component of nf-munr repressor; binds to the matrix attachment regions (MARs) (5' and 3') of the immunoglobulin heavy chain enhancer. Represses T-cell receptor (TCR) beta enhancer function by binding to MARbeta, an ATC-rich DNA sequence located upstream of the TCR beta enhancer. Belongs to the CUT homeobox family. 8 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:DNA-binding; Membrane protein, integral; Transcription factor Chromosomal Location of Human Ortholog: 7q22.1 Cellular Component: integral to Golgi membrane; nucleus; cytosol Molecular Function:sequence-specific DNA binding; chromatin binding Biological Process: regulation of transcription from RNA polymerase II promoter; intra-Golgi vesicle-mediated transport; transcription, DNA-dependent; multicellular organismal development; auditory receptor cell differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of dendrite morphogenesis; negative regulation of transcription from RNA polymerase II promoter; kidney development; lung development
NCBI Summary:	The protein encoded by this gene is a member of the homeodomain family of DNA binding proteins. It may regulate gene expression, morphogenesis, and differentiation and it may also play a role in the cell cycle progession. Several alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Feb 2011]
UniProt Code:	P39880
NCBI GenInfo Identifier:	296439379
NCBI Gene ID:	1523
NCBI Accession:	P39880.3
UniProt Secondary Accession:	P39880,Q6NYH4, Q75LE5, Q75MT2, Q75MT3, Q86UJ7, Q9UEV5 B3KV79, J3KQV9,
UniProt Related Accession:	P39880,Q13948
Molecular Weight:	77 kDa
NCBI Full Name:	Homeobox protein cut-like 1
NCBI Synonym Full Names:	cut-like homeobox 1
NCBI Official Symbol:	CUX1
NCBI Official Synonym Symbols:	CDP; CUX; p75; CASP; CDP1; COY1; Clox; p100; p110; p200; CUTL1; GOLIM6; CDP/Cut; Cux/CDP; Nbla10317
NCBI Protein Information:	protein CASP; cut homolog; homeobox protein cux-1; CCAAT displacement protein; golgi integral membrane protein 6; putative protein product of Nbla10317
UniProt Protein Name:	Homeobox protein cut-like 1
UniProt Synonym Protein Names:	CCAAT displacement protein; CDP; Homeobox protein cux-1
Protein Family:	Homeobox protein
UniProt Gene Name:	CUX1
UniProt Entry Name:	CUX1_HUMAN

	Western blot analysis of extracts of various cell lines, using CUX1 antibody (CAB2213) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of C6 cells using CUX1 antibody (CAB2213) at dilution of 1:100. Blue: DAPI for nuclear staining.