Name: Anti-CUL4B Antibody (CAB12696)
Brand: Cell Cycle Antibodies 1
SKU: CAB12696
Availability: OutOfStock

Antibody Name:	Anti-CUL4B Antibody
Antibody SKU:	CAB12696
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IP
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-152 of human CUL4B (NP_003579.3).

Application:	WB IP
Recommended Dilution:	WB 1:500 - 1:2000 IP 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	SH-SY5Y, HeLa, 293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-152 of human CUL4B (NP_003579.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MMSQ SSGS GDGN DDEA TTSK DGGF SSPS PSAA AAAQ EVRS ATDG NTST TPPT SAKK RKLN SSSS SSSN SSNE REDF DSTS SSSS TPPL QPRD SASP STSS FCLG VSVA ASSH VPIQ KKLR FEDT LEFV GFDA KMAE ESSS SSSS SSPT AATS
Gene ID:	8450
Uniprot:	Q13620
Cellular Location:	Nucleus
Calculated MW:	84kDa/102kDa/103kDa
Observed MW:	105kDa

Synonyms:

CUL4B, CUL-4B, MRXHF2, MRXS15, MRXSC, SFM2, cullin-4B

Background:

This gene is a member of the cullin family. The encoded protein forms a complex that functions as an E3 ubiquitin ligase and catalyzes the polyubiquitination of specific protein substrates in the cell. The protein interacts with a ring finger protein, and is required for the proteolysis of several regulators of DNA replication including chromatin licensing and DNA replication factor 1 and cyclin E. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	CUL4B iso1: Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit. CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, histone H2A, histone H3 and histone H4 in response to radiation- induced DNA damage. Targeted to UV damaged chromatin by DDB2 and may be important for DNA repair and DNA replication. Required for ubiquitination of cyclin E, and consequently, normal G1 cell cycle progression. Regulates the mammalian target-of-rapamycin (mTOR) pathway involved in control of cell growth, size and metabolism. Specific CUL4B regulation of the mTORC1-mediated pathway is dependent upon 26S proteasome function and requires interaction between CUL4B and MLST8. Defects in CUL4B are the cause of mental retardation syndromic X-linked Cabezas type (MRXC); also known as mental retardation syndromic X-linked type 15. A syndromic form of X-linked mental retardation characterized by severe intellectual deficit associated with short stature, craniofacial dysmorphism, small testes, muscle wasting in lower legs, kyphosis, joint hyperextensibility, pes cavus, small feet, and abnormalities of the toes. Additional neurologic manifestations include speech delay and impairment, tremor, seizures, gait ataxia, hyperactivity and decreased attention span. Belongs to the cullin family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cell cycle regulation Chromosomal Location of Human Ortholog: Xq23 Cellular Component: nucleoplasm; cytoplasm; plasma membrane Molecular Function:protein binding; ubiquitin protein ligase binding; damaged DNA binding Biological Process: ubiquitin-dependent protein catabolic process; positive regulation of protein catabolic process; cell cycle Disease: Mental Retardation, X-linked, With Short Stature, Hypogonadism, And Abnormal Gait
NCBI Summary:	This gene is a member of the cullin family. The encoded protein forms a complex that functions as an E3 ubiquitin ligase and catalyzes the polyubiquitination of specific protein substrates in the cell. The protein interacts with a ring finger protein, and is required for the proteolysis of several regulators of DNA replication including chromatin licensing and DNA replication factor 1 and cyclin E. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q13620
NCBI GenInfo Identifier:	296439468
NCBI Gene ID:	8450
NCBI Accession:	Q13620.4
UniProt Related Accession:	Q13620
Molecular Weight:	115kDa
NCBI Full Name:	Cullin-4B
NCBI Synonym Full Names:	cullin 4B
NCBI Official Symbol:	CUL4B
NCBI Official Synonym Symbols:	SFM2; MRXSC; CUL-4B; MRXHF2; MRXS15
NCBI Protein Information:	cullin-4B
UniProt Protein Name:	Cullin-4B
Protein Family:	Cullin
UniProt Gene Name:	CUL4B
UniProt Entry Name:	CUL4B_HUMAN

Western blot analysis of extracts of various cell lines, using CUL4B antibody (CAB12696) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.

Antibodies

Anti-CUL4B Antibody (CAB5400)

Anti-CUL4B Antibody (CAB6198)

CUL4B Antibody (PACO01901)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)