Name: Anti-CUL4A Antibody (CAB13911)
Brand: Cell Biology Antibodies 4
SKU: CAB13911
Availability: OutOfStock

Antibody Name:	Anti-CUL4A Antibody
Antibody SKU:	CAB13911
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 510-759 of human CUL4A (NP_001008895.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	HeLa, NCI-H460, HepG2, HL-60, HT-1080, Mouse brain, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 510-759 of human CUL4A (NP_001008895.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QHMQ NQSD SGPI DLTV NILT MGYW PTYT PMEV HLTP EMIK LQEV FKAF YLGK HSGR KLQW QTTL GHAV LKAE FKEG KKEF QVSL FQTL VLLM FNEG DGFS FEEI KMAT GIED SELR RTLQ SLAC GKAR VLIK SPKG KEVE DGDK FIFN GEFK HKLF RIKI NQIQ MKET VEEQ VSTT ERVF QDRQ YQID AAIV RIMK MRKT LGHN LLVS ELYN QLKF PVKP GDLK KRIE SLID RDYM ERDK DNPN QYHY VA
Gene ID:	8451
Uniprot:	Q13619
Cellular Location:
Calculated MW:	76kDa/87kDa
Observed MW:	80kDa/90kDa

Synonyms:	CUL4A, cullin-4A
Background:

UniProt Protein Function:	CUL4A: Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. In association with RBX1, DDB1 and DDB2 is required for histone H3 and histone H4 ubiquitination in response to ultraviolet and may be important for subsequent DNA repair. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. In association with DDB1 and SKP2 probably is involved in ubiquitination of p27Kip1/p27kip. Is involved in ubiquitination of HOXA9. Component of multiple DCX (DDB1-CUL4-X-box) E3 ubiquitin- protein ligase complexes that seem to consist of DDB1, CUL4A or CUL4B, RBX1 and a variable substrate recognition component which seems to belong to a protein family described as DCAF (Ddb1- and Cul4-associated factor) or CDW (CUL4-DDB1-associated WD40-repeat) proteins. Component of the CSA complex (DCX(ERCC8) complex) containing ERCC8, RBX1, DDB1 and CUL4A; the CSA complex interacts with RNA polymerase II; upon UV irradiation it interacts with the COP9 signalosome and preferentially with the hyperphosphorylated form of RNA polymerase II. Component of the DCX(DET1-COP1) complex with the substrate recognition component DET1 and COP1. Component of the DCX(DDB2) complex with the substrate recognition component DDB2. Component of the DCX(DTL) complex with the putative substrate recognition component DTL. Interacts with DDB1, RBX1, RNF7, CTD1, TIP120A/CAND1, SKP2, p27Kip1, MDM2, TP53 and HOXA9. Interacts with DDB2; the interactions with DDB2 and CAND1 are mutually exclusive. Interacts with VPRBP, DTL, DDA1, DCAF6, DCAF4, DCAF16, DCAF17, DET1, WDTC1, DCAF5, DCAF11, WDR24A, RFWD2, PAFAH1B1, ERCC8, GRWD1, FBXW5, RBBP7, GNB2, WSB1, WSB2, NUP43, PWP1, FBXW8, ATG16L1, KATNB1, RBBP4, RBBP5 and DCAF8. May interact with WDR26, WDR51B, SNRNP40, WDR61, WDR76, WDR5. Can self- associate. Interacts with Epstein-Barr virus BPLF1. Belongs to the cullin family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 13q34 Cellular Component: nucleoplasm Molecular Function:protein binding; ubiquitin protein ligase binding Biological Process: cell cycle arrest; DNA damage response, detection of DNA damage; G1/S transition of mitotic cell cycle; negative regulation of cell proliferation; nucleotide-excision repair, DNA damage recognition; nucleotide-excision repair, DNA duplex unwinding; nucleotide-excision repair, DNA incision; nucleotide-excision repair, DNA incision, 3'-to lesion; nucleotide-excision repair, DNA incision, 5'-to lesion; nucleotide-excision repair, preincision complex assembly; nucleotide-excision repair, preincision complex stabilization; protein ubiquitination during ubiquitin-dependent protein catabolic process; response to DNA damage stimulus; transcription-coupled nucleotide-excision repair
NCBI Summary:	CUL4A is the ubiquitin ligase component of a multimeric complex involved in the degradation of DNA damage-response proteins (Liu et al., 2009 [PubMed 19481525]).[supplied by OMIM, Oct 2009]
UniProt Code:	Q13619
NCBI GenInfo Identifier:	108936013
NCBI Gene ID:	8451
NCBI Accession:	Q13619.3
UniProt Secondary Accession:	Q13619,O75834, Q589T6, Q5TC62, Q6UP08, Q9UP17, A2A2W2
UniProt Related Accession:	Q13619
Molecular Weight:	76,821 Da
NCBI Full Name:	Cullin-4A
NCBI Synonym Full Names:	cullin 4A
NCBI Official Symbol:	CUL4A
NCBI Protein Information:	cullin-4A
UniProt Protein Name:	Cullin-4A
Protein Family:	Cullin
UniProt Gene Name:	CUL4A
UniProt Entry Name:	CUL4A_HUMAN

Western blot analysis of extracts of various cell lines, using CUL4A antibody (CAB13911) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

Anti-CUL4A Antibody (CAB2882)

Anti-Cullin4A Antibody (CAB5064)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)