Immunology Antibodies 2
Anti-CUL2 Antibody (CAB5308)
- SKU:
- CAB5308
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-CUL2 Antibody |
| Antibody SKU: | CAB5308 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 40-310 of human CUL2 (NP_001185708.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human |
| Positive Samples: | PC-3, K-562, HL-60, 293T, OVCAR3 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 40-310 of human CUL2 (NP_001185708.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LEYV ERAT WNDR FSDI YALC VAYP EPLG ERLY TETK IFLE NHVR HLHK RVLE SEEQ VLVM YHRY WEEY SKGA DYMD CLYR YLNT QFIK KNKL TEAD LQYG YGGV DMNE PLME IGEL ALDM WRKL MVEP LQAI LIRM LLRE IKND RGGE DPNQ KVIH GVIN SFVH VEQY KKKF PLKF YQEI FESP FLTE TGEY YKQE ASNL LQES NCSQ YMEK VLGR LKDE EIRC RKYL HPSS YTKV IHEC QQRM VADH LQFL HAEC HNII RQEK KNDM ANM |
| Gene ID: | 8453 |
| Uniprot: | Q13617 |
| Cellular Location: | |
| Calculated MW: | 86kDa/89kDa |
| Observed MW: | 87kDa |
| Synonyms: | CUL2, cullin-2 |
| Background: |
| UniProt Protein Function: | CUL2: Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin- conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF). Belongs to the cullin family. |
| UniProt Protein Details: | Protein type:Ubiquitin conjugating system; Cell cycle regulation Chromosomal Location of Human Ortholog: 10p11.21 Cellular Component: Cul2-RING ubiquitin ligase complex; nucleoplasm; cytosol Molecular Function:protein binding; ubiquitin protein ligase binding Biological Process: ubiquitin-dependent protein catabolic process; negative regulation of cell proliferation; viral reproduction; protein ubiquitination; cell cycle arrest; G1/S transition of mitotic cell cycle |
| UniProt Code: | Q13617 |
| NCBI GenInfo Identifier: | 19863260 |
| NCBI Gene ID: | 8453 |
| NCBI Accession: | Q13617.2 |
| UniProt Secondary Accession: | Q13617,O00200, Q5T2B6, Q9UNF9, B3KT95, B7Z6K8, D3DRY6 G3V1S2, |
| UniProt Related Accession: | Q13617 |
| Molecular Weight: | 745 |
| NCBI Full Name: | Cullin-2 |
| NCBI Synonym Full Names: | cullin 2 |
| NCBI Official Symbol: | CUL2 |
| NCBI Protein Information: | cullin-2; CUL-2 |
| UniProt Protein Name: | Cullin-2 |
| Protein Family: | Cullin |
| UniProt Gene Name: | CUL2 |
| UniProt Entry Name: | CUL2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CUL2 antibody (CAB5308) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of A-549 cells using CUL2 antibody (CAB5308). Blue: DAPI for nuclear staining. |
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