Epigenetics & Nuclear Signaling Antibodies 1

Anti-CTU2 Antibody (CAB13003)

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SKU:
CAB13003
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-CTU2 Antibody
Antibody SKU:CAB13003
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 186-515 of human CTU2 (NP_001012777.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:A-431, Jurkat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 186-515 of human CTU2 (NP_001012777.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:HVLG AGGG PGPT QGEE QPPQ PPLD PQNL ARPP APAQ TEAL SQLF CSVR TLTA KEEL LQTL RTHL ILHM ARAH GYSK VMTG DSCT RLAI KLMT NLAL GRGA FLAW DTGF SDER HGDV VVVR PMRD HTLK EVAF YNRL FSVP SVFT PAVD TKAP EKAS IHRL MEAF ILRL QTQF PSTV STVY RTSE KLVK GPRD GPAA GDSG PRCL LCMC ALDV DAAD SATA FGAQ TSSR LSQM QSPI PLTE TRTP PGPC CSPG VGWA QRCG QGAC RRED PQAC IEEQ LCYS CRVN MKDL PSLD PLPP YILA EAQL RTQR AWGL QEIR DCLI EDSD DEAG QS
Gene ID:348180
Uniprot:Q2VPK5
Cellular Location:Cytoplasm
Calculated MW:46kDa/52kDa/56kDa
Observed MW:56kDa
Synonyms:CTU2, C16orf84, NCS2, UPF0432
Background:This gene encodes a protein which is involved in the post-transcriptional modification of transfer RNAs (tRNAs). The encoded protein plays a role in thiolation of uridine residue present at the wobble position in a subset of tRNAs, resulting in enhanced codon reading accuracy. Alternative splicing results in multiple transcript variants encoding different isoforms.
UniProt Protein Function:CTU2: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by forming a heterodimer with CTU1/ATPBD3 that ligates sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Belongs to the CTU2/NCS2 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:RNA-binding

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: protein complex; mitochondrion; cytoplasm

Molecular Function:protein binding; tRNA binding; nucleotidyltransferase activity

Biological Process: protein urmylation; tRNA wobble uridine modification

UniProt Code:Q2VPK5
NCBI GenInfo Identifier:121941955
NCBI Gene ID:348180
NCBI Accession:Q2VPK5.1
UniProt Secondary Accession:Q2VPK5,Q0P511, Q66K78, Q6P4C8, Q86SV4, B2RXK0,
UniProt Related Accession:Q2VPK5
Molecular Weight:52,467 Da
NCBI Full Name:Cytoplasmic tRNA 2-thiolation protein 2
NCBI Synonym Full Names:cytosolic thiouridylase subunit 2 homolog (S. pombe)
NCBI Official Symbol:CTU2  
NCBI Official Synonym Symbols:NCS2; UPF0432; C16orf84  
NCBI Protein Information:cytoplasmic tRNA 2-thiolation protein 2
UniProt Protein Name:Cytoplasmic tRNA 2-thiolation protein 2
UniProt Gene Name:CTU2  
UniProt Entry Name:CTU2_HUMAN
Anti-CTU2 Antibody (CAB13003)Western blot analysis of extracts of various cell lines, using CTU2 antibody (CAB13003) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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