Cell Biology Antibodies 5

Anti-CTTN Antibody (CAB15054)

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SKU:
CAB15054
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-CTTN Antibody
Antibody SKU:CAB15054
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human CTTN (NP_612632.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human CTTN (NP_612632.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MWKA SAGH AVSI AQDD AGAD DWET DPDF VNDV SEKE QRWG AKTV QGSG HQEH INIH KLRE NVFQ EHQT LKEK ELET GPKA SHGY GGKF GVEQ DRMD KSAV GHEY QSKL SKHC SQVD SVRG FGGK FGVQ MDRV DQSA VGFE YQGK TEKH ASQK DYSS GFGG KYGV QADR VDKS AVGF DYQG KTEK HESQ RDYS KGFG GKYG
Gene ID:2017
Uniprot:Q14247
Cellular Location:Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic side, Membrane, Peripheral membrane protein, cell cortex, clathrin-coated pit, cytoskeleton, dendrite, dendritic spine, focal adhesion, lamellipodium, podosome, ruffle
Calculated MW:57kDa/61kDa/70kDa
Observed MW:85kDa
Synonyms:CTTN, EMS1, cortactin
Background:This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene.
UniProt Protein Function:Cortactin: a cytoskeletal protein that that is involved in coordinating actin reorganization during cell movement. Localizes at the leading edge of lamellipodia during cell migration. Its amino-terminal acidic domain associates with the Arp2/3 and WASP complex at F-actin branches. The central region of the protein contains six repeats of 37 amino acids that are important in F-actin binding and cross-linking. The carboxy-terminus contains a proline-rich region and an SH3 domain that can interact with numerous scaffolding proteins, such as CortBP1 and Shank3. Cortactin may play a role in the organization of transmembrane receptors at postsynaptic densities (PSD) and tight junctions by linking scaffolding proteins to the actin network. It is degraded in a caspase-dependent manner during apoptosis. It is overexpressed in numerous cancers with poor patient prognosis. It may contribute to tumor cell invasion and metastasis. Two splice variant isoforms have been identified.
UniProt Protein Details:

Protein type:Actin-binding; Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: actin filament; cell cortex; coated pit; cortical cytoskeleton; cytoplasm; cytoskeleton; focal adhesion; Golgi apparatus; growth cone; intracellular membrane-bound organelle; lamellipodium; plasma membrane; ruffle; voltage-gated potassium channel complex

Molecular Function:profilin binding; protein binding

Biological Process: actin cytoskeleton reorganization; cell motility involved in cell locomotion; focal adhesion formation; intracellular protein transport; neurite morphogenesis; positive regulation of actin filament polymerization; positive regulation of smooth muscle contraction; receptor-mediated endocytosis; regulation of axon extension

NCBI Summary:This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene. [provided by RefSeq, May 2010]
UniProt Code:Q14247
NCBI GenInfo Identifier:215273892
NCBI Gene ID:2017
NCBI Accession:Q14247.2
UniProt Secondary Accession:Q14247,Q8N707, Q96H99,
UniProt Related Accession:Q14247,AAB26248
Molecular Weight:57,467 Da
NCBI Full Name:Src substrate cortactin
NCBI Synonym Full Names:cortactin
NCBI Official Symbol:CTTN  
NCBI Official Synonym Symbols:EMS1  
NCBI Protein Information:src substrate cortactin
UniProt Protein Name:Src substrate cortactin
UniProt Synonym Protein Names:Amplaxin; Oncogene EMS1
Protein Family:CTTNBP2 N-terminal-like protein
UniProt Gene Name:CTTN  
UniProt Entry Name:SRC8_HUMAN
Anti-CTTN Antibody (CAB15054)Western blot analysis of extracts of various cell lines, using CTTN antibody (CAB15054) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Anti-CTTN Antibody (CAB15054)Immunohistochemistry of paraffin-embedded human lung cancer using CTTN antibody (CAB15054) at dilution of 1:100 (40x lens).
Anti-CTTN Antibody (CAB15054)Immunohistochemistry of paraffin-embedded human mammary cancer using CTTN antibody (CAB15054) at dilution of 1:100 (40x lens).
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