Name: Anti-CTSH Antibody (CAB5368)
Brand: Cell Biology Antibodies 9
SKU: CAB5368
Availability: OutOfStock

Antibody Name:	Anti-CTSH Antibody
Antibody SKU:	CAB5368
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 23-335 of human CTSH (NP_004381.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	K-562, A-549, HT-29

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 23-335 of human CTSH (NP_004381.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AELC VNSL EKFH FKSW MSKH RKTY STEE YHHR LQTF ASNW RKIN AHNN GNHT FKMA LNQF SDMS FAEI KHKY LWSE PQNC SATK SNYL RGTG PYPP SVDW RKKG NFVS PVKN QGAC GSCW TFST TGAL ESAI AIAT GKML SLAE QQLV DCAQ DFNN HGCQ GGLP SQAF EYIL YNKG IMGE DTYP YQGK DGYC KFQP GKAI GFVK DVAN ITIY DEEA MVEA VALY NPVS FAFE VTQD FMMY RTGI YSST SCHK TPDK VNHA VLAV GYGE KNGI PYWI VKNS WGPQ WGMN GYFL IERG KNMC GLAA CASY PIPL V
Gene ID:	1512
Uniprot:	P09668
Cellular Location:	Lysosome
Calculated MW:	37kDa
Observed MW:	32kDa

Synonyms:

CTSH, ACC-4, ACC-5, ACC4, ACC5, CPSB

Background:

The protein encoded by this gene is a lysosomal cysteine proteinase important in the overall degradation of lysosomal proteins. It is composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. The encoded protein, which belongs to the peptidase C1 protein family, can act both as an aminopeptidase and as an endopeptidase. Increased expression of this gene has been correlated with malignant progression of prostate tumors. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	CTSH: Important for the overall degradation of proteins in lysosomes. Belongs to the peptidase C1 family.
UniProt Protein Details:	Protein type:EC 3.4.22.16; Protease Chromosomal Location of Human Ortholog: 15q25.1 Cellular Component: cytosol; extracellular space; lysosome Molecular Function:aminopeptidase activity; caspase activator activity; cysteine-type endopeptidase activity; cysteine-type peptidase activity; endopeptidase activity; HLA-A specific activating MHC class I receptor activity; peptidase activity; protein binding; serine-type endopeptidase activity Biological Process: adaptive immune response; antigen processing and presentation; cellular protein metabolic process; immune response-regulating signal transduction; membrane protein proteolysis; metanephros development; negative regulation of apoptosis; positive regulation of angiogenesis; positive regulation of cell migration; positive regulation of cell proliferation; protein destabilization; proteolysis; proteolysis involved in cellular protein catabolic process; response to retinoic acid; surfactant homeostasis; T cell mediated cytotoxicity; zymogen activation
NCBI Summary:	The protein encoded by this gene is a lysosomal cysteine proteinase important in the overall degradation of lysosomal proteins. It is composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. The encoded protein, which belongs to the peptidase C1 protein family, can act both as an aminopeptidase and as an endopeptidase. Increased expression of this gene has been correlated with malignant progression of prostate tumors. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]
UniProt Code:	P09668
NCBI GenInfo Identifier:	288558851
NCBI Gene ID:	1512
NCBI Accession:	P09668.4
UniProt Secondary Accession:	P09668,Q96NY6, Q9BUM7, B2RBK0,
UniProt Related Accession:	P09668
Molecular Weight:	37,394 Da
NCBI Full Name:	Pro-cathepsin H
NCBI Synonym Full Names:	cathepsin H
NCBI Official Symbol:	CTSH
NCBI Official Synonym Symbols:	ACC4; ACC5; CPSB; ACC-4; ACC-5
NCBI Protein Information:	pro-cathepsin H
UniProt Protein Name:	Pro-cathepsin H
Protein Family:	Pro-cathepsin
UniProt Gene Name:	CTSH
UniProt Entry Name:	CATH_HUMAN

Western blot analysis of extracts of various cell lines, using CTSH antibody (CAB5368) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

CTSH Antibody (PACO00020)

CTSH Antibody (PACO03539)

CTSH Antibody (PACO03540)

CTSH Antibody (PACO08672)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)