KO Validated Antibodies 2
Anti-CTCF Antibody [KO Validated] (CAB19588)
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![Anti-CTCF Antibody [KO Validated] (CAB19588)](https://cdn11.bigcommerce.com/s-rd6ounxcu2/images/stencil/608x608/products/55946/61128/anti-ctcf-antibody-ko-validated-cab19588__23370__57834.1706533717.jpg?c=1 "Anti-CTCF Antibody [KO Validated] (CAB19588)")
- SKU:
- CAB19588
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IF
- Applications:
- IP
- Applications:
- ChIP
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
![Anti-CTCF Antibody [KO Validated] (CAB19588)](https://cdn11.bigcommerce.com/s-rd6ounxcu2/images/stencil/1280x1280/products/55946/61128/anti-ctcf-antibody-ko-validated-cab19588__23370__57834.1706533717.jpg?c=1&imbypass=on "Anti-CTCF Antibody [KO Validated] (CAB19588)")
Description
| Antibody Name: | Anti-CTCF Antibody [KO Validated] |
| Antibody SKU: | CAB19588 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF IP ChIP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthesized peptide derived from human CTCF. |
| Application: | WB IF IP ChIP |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 ChIP 1:20 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Jurkat, HeLa, Mouse lung, 293T |
| Immunogen: | A synthesized peptide derived from human CTCF. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 10664 |
| Uniprot: | P49711 |
| Cellular Location: | Chromosome, Nucleus, centromere, nucleoplasm |
| Calculated MW: | 140kDa |
| Observed MW: | 140KDa |
 | Western blot analysis of extracts of various cell lines, using CTCF antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. |
 | Immunofluorescence analysis of C6 cells using CTCF antibody (A19588). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH-3T3 cells using CTCF antibody (A19588). Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug CTCF antibody at a dilition of 1:1000. |
 | Chromatin immunoprecipitation analysis of extracts of 293T cells, using CTCF antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input. |
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