KO Validated Antibodies 2

Anti-CTCF Antibody (CAB18627)[KO Validated]

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SKU:
CAB18627
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-CTCF Antibody [KO Validated]
Antibody SKU:CAB18627
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human CTCF.
Application:WB IHC IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T, NIH/3T3, C6
Immunogen:Recombinant protein of human CTCF.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:10664
Uniprot:P49711
Cellular Location:Chromosome, Nucleus, centromere, nucleoplasm
Calculated MW:45kDa/82kDa
Observed MW:140kDa
Western blot - [KO Validated] CTCF Rabbit pAb Western blot analysis of extracts from normal (control) and CTCF knockout (KO) 293T cells, using CTCF antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Western blot - [KO Validated] CTCF Rabbit pAb Western blot analysis of extracts of various cell lines, using CTCF antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry - [KO Validated] CTCF Rabbit pAb Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] CTCF Rabbit pAb at dilution of 1:250 (40x lens).
Immunoprecipitation - [KO Validated] CTCF Rabbit pAb Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug CTCF antibody at a dilition of 1:500.
Chromatin Immunoprecipitation - [KO Validated] CTCF Rabbit pAb Chromatin immunoprecipitation analysis of extracts of HeLa cells, using CTCF antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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