Epigenetics & Nuclear Signaling Antibodies 1
Anti-CSTF3 Antibody (CAB11665)
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- SKU:
- CAB11665
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-CSTF3 Antibody |
| Antibody SKU: | CAB11665 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-103 of human CSTF3 (NP_001028677.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse liver, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-103 of human CSTF3 (NP_001028677.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSGD GATE QAAE YVPE KVKK AEKK LEEN PYDL DAWS ILIR EAQN QPID KARK TYER LVAQ FPSS GRFW KLYI EAEV TILF YFFL YQYC SIHC SDRK QVRN IAN |
| Gene ID: | 1479 |
| Uniprot: | Q12996 |
| Cellular Location: | Nucleus |
| Calculated MW: | 4kDa/12kDa/82kDa |
| Observed MW: | 77kDa |
| Synonyms: | CSTF3, CSTF-77 |
| Background: | The protein encoded by this gene is one of three (including CSTF1 and CSTF2) cleavage stimulation factors that combine to form the cleavage stimulation factor complex (CSTF). This complex is involved in the polyadenylation and 3' end cleavage of pre-mRNAs. The encoded protein functions as a homodimer and interacts directly with both CSTF1 and CSTF2 in the CSTF complex. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| UniProt Protein Function: | CstF-77: One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:RNA splicing; RNA-binding Chromosomal Location of Human Ortholog: 11p13 Cellular Component: nucleoplasm; nucleus Molecular Function:mRNA binding; protein binding; RNA binding Biological Process: mRNA 3'-end processing; mRNA cleavage; mRNA polyadenylation; nuclear mRNA splicing, via spliceosome; termination of RNA polymerase II transcription |
| NCBI Summary: | The protein encoded by this gene is one of three (including CSTF1 and CSTF2) cleavage stimulation factors that combine to form the cleavage stimulation factor complex (CSTF). This complex is involved in the polyadenylation and 3' end cleavage of pre-mRNAs. The encoded protein functions as a homodimer and interacts directly with both CSTF1 and CSTF2 in the CSTF complex. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q12996 |
| NCBI GenInfo Identifier: | 71153231 |
| NCBI Gene ID: | 1479 |
| NCBI Accession: | Q12996.1 |
| UniProt Secondary Accession: | Q12996,Q32P22, Q96FQ8, Q96QD6, Q96QK4, A8K471, D3DR04 E9PB40, |
| UniProt Related Accession: | Q12996 |
| Molecular Weight: | 4,952 Da |
| NCBI Full Name: | Cleavage stimulation factor subunit 3 |
| NCBI Synonym Full Names: | cleavage stimulation factor subunit 3 |
| NCBI Official Symbol: | CSTF3 |
| NCBI Official Synonym Symbols: | CSTF-77 |
| NCBI Protein Information: | cleavage stimulation factor subunit 3 |
| UniProt Protein Name: | Cleavage stimulation factor subunit 3 |
| UniProt Synonym Protein Names: | CF-1 77 kDa subunit; Cleavage stimulation factor 77 kDa subunit; CSTF 77 kDa subunit; CstF-77 |
| Protein Family: | Cleavage stimulation factor |
| UniProt Gene Name: | CSTF3 |
| UniProt Entry Name: | CSTF3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CSTF3 antibody (CAB11665) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 50s. |
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