Epigenetics & Nuclear Signaling Antibodies 2

Anti-CSTF1 Antibody (CAB5915)

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SKU:
CAB5915
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-CSTF1 Antibody
Antibody SKU:CAB5915
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human CSTF1 (NP_001315.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:U2OS, MCF7, SW480, Mouse thymus, Rat thymus
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human CSTF1 (NP_001315.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MYRT KVGL KDRQ QLYK LIIS QLLY DGYI SIAN GLIN EIKP QSVC APSE QLLH LIKL GMEN DDTA VQYA IGRS DTVA PGTG IDLE FDAD VQTM SPEA SEYE TCYV TSHK GPCR VATY SRDG QLIA TGSA DASI KILD TERM LAKS AMPI EVMM NETA QQNM ENHP VIRT LYDH VDEV TCLA FHPT EQIL ASGS RDYT LKLF DYSK PSAK RAFK YIQE AEML
Gene ID:1477
Uniprot:Q05048
Cellular Location:Nucleus
Calculated MW:48kDa
Observed MW:50kDa
Synonyms:CSTF1, CstF-50, CstFp50
Background:This gene encodes one of three subunits which combine to form cleavage stimulation factor (CSTF). CSTF is involved in the polyadenylation and 3'end cleavage of pre-mRNAs. Similar to mammalian G protein beta subunits, this protein contains transducin-like repeats. Several transcript variants with different 5' UTR, but encoding the same protein, have been found for this gene.
UniProt Protein Function:One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. May be responsible for the interaction of CSTF with other factors to form a stable complex on the pre-mRNA.
NCBI Summary:This gene encodes one of three subunits which combine to form cleavage stimulation factor (CSTF). CSTF is involved in the polyadenylation and 3'end cleavage of pre-mRNAs. Similar to mammalian G protein beta subunits, this protein contains transducin-like repeats. Several transcript variants with different 5' UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:Q05048
NCBI GenInfo Identifier:461848
NCBI Gene ID:1477
NCBI Accession:Q05048.1
UniProt Secondary Accession:Q05048,Q5QPD8,
UniProt Related Accession:Q05048
Molecular Weight:48,358 Da
NCBI Full Name:Cleavage stimulation factor subunit 1
NCBI Synonym Full Names:cleavage stimulation factor subunit 1
NCBI Official Symbol:CSTF1  
NCBI Official Synonym Symbols:CstF-50; CstFp50  
NCBI Protein Information:cleavage stimulation factor subunit 1
UniProt Protein Name:Cleavage stimulation factor subunit 1
UniProt Synonym Protein Names:CF-1 50 kDa subunit; Cleavage stimulation factor 50 kDa subunit; CSTF 50 kDa subunit; CstF-50
Protein Family:Cleavage stimulation factor
UniProt Gene Name:CSTF1  
Anti-CSTF1 Antibody (CAB5915)Western blot analysis of extracts of various cell lines, using CSTF1 antibody (CAB5915) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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