Cell Biology Antibodies 2

Anti-CST8 Antibody (CAB1224)

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SKU:
CAB1224
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-CST8 Antibody
Antibody SKU:CAB1224
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 53-142 of human CST8 (NP_005483.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:Mouse testis, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 53-142 of human CST8 (NP_005483.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MQEY NKES EDKY VFLV VKTL QAQL QVTN LLEY LIDV EIAR SDCR KPLS TNEI CAIQ ENSK LKRK LSCS FLVG ALPW NGEF TVME KKCE DA
Gene ID:10047
Uniprot:O60676
Cellular Location:Secreted
Calculated MW:16kDa
Observed MW:27kDa
Synonyms:CST8, CRES, CTES5
Background:The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a protein similar to type 2 cystatins. The encoded protein exhibits highly tissue-specific expression in the reproductive tract, suggesting implicit roles in reproduction. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:CST8: Performs a specialized role during sperm development and maturation. Belongs to the cystatin family.
UniProt Protein Details:

Protein type:Inhibitor; Secreted; Cell surface; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20p11.21

Cellular Component: cell surface; cytoplasm; extracellular region

Molecular Function:cysteine protease inhibitor activity

NCBI Summary:The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a protein similar to type 2 cystatins. The encoded protein exhibits highly tissue-specific expression in the reproductive tract, suggesting implicit roles in reproduction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
UniProt Code:O60676
NCBI GenInfo Identifier:6225205
NCBI Gene ID:10047
NCBI Accession:O60676.1
UniProt Secondary Accession:O60676,Q2M2X6,
UniProt Related Accession:O60676
Molecular Weight:142
NCBI Full Name:Cystatin-8
NCBI Synonym Full Names:cystatin 8 (cystatin-related epididymal specific)
NCBI Official Symbol:CST8  
NCBI Official Synonym Symbols:CRES; CTES5  
NCBI Protein Information:cystatin-8; cystatin-related epididymal spermatogenic protein
UniProt Protein Name:Cystatin-8
UniProt Synonym Protein Names:Cystatin-related epididymal spermatogenic protein
Protein Family:Cystatin
UniProt Gene Name:CST8  
UniProt Entry Name:CST8_HUMAN
Anti-CST8 Antibody (CAB1224)Western blot analysis of extracts of various cell lines, using CST8 antibody (CAB1224) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-CST8 Antibody (CAB1224)Immunofluorescence analysis of HeLa cells using CST8 antibody (CAB1224). Blue: DAPI for nuclear staining.
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