Name: Anti-CSPG4 Antibody (CAB3592)[KO Validated]
Brand: KO Validated Antibodies 1
SKU: CAB3592
Availability: OutOfStock

Antibody Name:	Anti-CSPG4 Antibody [KO Validated]
Antibody SKU:	CAB3592
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1950-2210 of human CSPG4 (NP_001888.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-375, HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1950-2210 of human CSPG4 (NP_001888.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LEVP QALG RSSL SQQQ LRVV SDRE EPEA AYRL IQGP QYGH LLVG GRPT SAFS QFQI DQGE VVFA FTNF SSSH DHFR VLAL ARGV NASA VVNV TVRA LLHV WAGG PWPQ GATL RLDP TVLD AGEL ANRT GSVP RFRL LEGP RHGR VVRV PRAR TEPG GSQL VEQF TQQD LEDG RLGL EVGR PEGR APGP AGDS LTLE LWAQ GVPP AVAS LDFA TEPY NAAR PYSV ALLS VPEA ARTE AGKP ESST PTGE PGPM ASSP EPAV A
Gene ID:	1464
Uniprot:	Q6UVK1
Cellular Location:	Apical cell membrane, Cell projection, Extracellular side, Single-pass type I membrane protein, lamellipodium membrane
Calculated MW:	250kDa
Observed MW:	320kDa

Synonyms:	CSPG4, HMW-MAA, MCSP, MCSPG, MEL-CSPG, MSK16, NG2
Background:	A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.

UniProt Protein Function:	NG2: a transmembrane chondrotin sulfate proteoglycan expressed on several types of immature progenitor cells and human malignant melanoma cells. Plays a role in cell proliferation and motility of endothelial cells during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. May regulate MPP16-dependent degradation and invasion of type I collagen participating to melanoma cells invasion properties. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through Cdc42, ACK1 and P130Cas. May activate FAK and ERK signaling cascades. PKC-alpha-mediated NG2 phosphorylation may be a key step for initiating cell polarization and motility.
UniProt Protein Details:	Protein type:Cell adhesion; Membrane protein, integral Chromosomal Location of Human Ortholog: 15q24.2 Cellular Component: lysosomal lumen; cell surface; focal adhesion; integral to plasma membrane; Golgi lumen; apical plasma membrane; extracellular region Molecular Function:signal transducer activity; protein kinase binding Biological Process: chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; activation of MAPK activity; tissue remodeling; pathogenesis; glial cell migration; dermatan sulfate biosynthetic process; chondroitin sulfate metabolic process; cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; carbohydrate metabolic process; chondroitin sulfate catabolic process; angiogenesis; transmembrane receptor protein tyrosine kinase signaling pathway
NCBI Summary:	A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. [provided by RefSeq, Jul 2008]
UniProt Code:	Q6UVK1
NCBI GenInfo Identifier:	296434468
NCBI Gene ID:	1464
NCBI Accession:	Q6UVK1.2
UniProt Related Accession:	Q6UVK1
Molecular Weight:	251kDa
NCBI Full Name:	Chondroitin sulfate proteoglycan 4
NCBI Synonym Full Names:	chondroitin sulfate proteoglycan 4
NCBI Official Symbol:	CSPG4
NCBI Official Synonym Symbols:	NG2; MCSP; MCSPG; MSK16; CSPG4A; HMW-MAA; MEL-CSPG
NCBI Protein Information:	chondroitin sulfate proteoglycan 4
UniProt Protein Name:	Chondroitin sulfate proteoglycan 4
UniProt Synonym Protein Names:	Chondroitin sulfate proteoglycan NG2; Melanoma chondroitin sulfate proteoglycan; Melanoma-associated chondroitin sulfate proteoglycan
Protein Family:	Protein new-glue
UniProt Gene Name:	CSPG4
UniProt Entry Name:	CSPG4_HUMAN

	Western blot analysis of extracts of various cell lines, using CSPG4 antibody (CAB3592) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Western blot analysis of extracts from normal (control) and CSPG4 knockout (KO) HeLa cells, using CSPG4 antibody (CAB3592) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.