Antibody Name:	Anti-CSNK1E Antibody [KO Validated]
Antibody SKU:	CAB1796
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 247-416 of human CSNK1E (NP_689407.1).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, 293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 247-416 of human CSNK1E (NP_689407.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EFST YLNF CRSL RFDD KPDY SYLR QLFR NLFH RQGF SYDY VFDW NMLK FGAA RNPE DVDR ERRE HERE ERMG QLRG SATR ALPP GPPT GATA NRLR SAAE PVAS TPAS RIQP AGNT SPRA ISRV DRER KVSM RLHR GAPA NVSS SDLT GRQE VSRI PASQ TSVP FDHL GK
Gene ID:	1454
Uniprot:	P49674
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	47kDa
Observed MW:	47kDa

Synonyms:

CSNK1E, CKIepsilon, HCKIE

Background:

The protein encoded by this gene is a serine/threonine protein kinase and a member of the casein kinase I protein family, whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein is found in the cytoplasm as a monomer and can phosphorylate a variety of proteins, including itself. This protein has been shown to phosphorylate period, a circadian rhythm protein. Two transcript variants encoding the same protein have been found for this gene.

UniProt Protein Function:	CK1E: an ubiquitous protein kinase of the CK1 family. Together with Dvl-1 and Frat-1 activate the Wnt signaling pathway. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. May play a role in cell cycle progression. Two splice variant isoforms have been described. Component of the circadian core oscillator, which includes the CRY proteins, CLOCK, or NPAS2, BMAL1 or BMAL2, CK1-D and/or CK1-E, TIMELESS and the PER proteins. Interacts directly with PER1 and PER2 which may lead to their degradation. Interacts with SOCS3. Mutations in hamster and Drosophila orthologs have circadian rhythm phenotypes, and the circadian gene period (per) is a substrate in both human and fly. A coding SNP variant in human, which increases CK1 activity, is negatively associated with circadian disorder. LOF mutations and LOH seen in mammary ductal carcinoma.
UniProt Protein Details:	Protein type:Protein kinase, CK1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; CK1 group; CK1 family Chromosomal Location of Human Ortholog: 22q13.1 Cellular Component: cytoplasm; nucleus; cytosol; ribonucleoprotein complex Molecular Function:protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity Biological Process: circadian rhythm; Wnt receptor signaling pathway; negative regulation of Wnt receptor signaling pathway; organelle organization and biogenesis; endocytosis; DNA repair; signal transduction; regulation of circadian rhythm; protein amino acid phosphorylation; regulation of cell shape; peptidyl-serine phosphorylation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; circadian regulation of gene expression; mitotic cell cycle; G2/M transition of mitotic cell cycle
NCBI Summary:	The protein encoded by this gene is a serine/threonine protein kinase and a member of the casein kinase I protein family, whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein is found in the cytoplasm as a monomer and can phosphorylate a variety of proteins, including itself. This protein has been shown to phosphorylate period, a circadian rhythm protein. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Feb 2014]
UniProt Code:	P49674
NCBI GenInfo Identifier:	1346369
NCBI Gene ID:	1454
NCBI Accession:	P49674.1
UniProt Related Accession:	P49674
Molecular Weight:	416
NCBI Full Name:	Casein kinase I isoform epsilon
NCBI Synonym Full Names:	casein kinase 1, epsilon
NCBI Official Symbol:	CSNK1E
NCBI Official Synonym Symbols:	HCKIE; CKIepsilon
NCBI Protein Information:	casein kinase I isoform epsilon; CKIe; CKI-epsilon
UniProt Protein Name:	Casein kinase I isoform epsilon
Protein Family:	Casein kinase
UniProt Gene Name:	CSNK1E
UniProt Entry Name:	KC1E_HUMAN

	Western blot analysis of extracts of various cell lines, using CSNK1E antibody (CAB1796) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Western blot analysis of extracts from normal (control) and CSNK1E knockout (KO) HeLa cells, using CSNK1E antibody (CAB1796) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded rat pancreas using CSNK1E Antibody (CAB1796) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using CSNK1E Antibody (CAB1796) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of HeLa cells using CSNK1E antibody (CAB1796). Blue: DAPI for nuclear staining.