Antibody Name:	Anti-CSK Antibody [KO Validated]
Antibody SKU:	CAB0735
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 241-450 of human CSK (NP_004374.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, Jurkat, Raji, Mouse lung, Mouse spleen, Mouse thymus, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 241-450 of human CSK (NP_004374.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TQLR HSNL VQLL GVIV EEKG GLYI VTEY MAKG SLVD YLRS RGRS VLGG DCLL KFSL DVCE AMEY LEGN NFVH RDLA ARNV LVSE DNVA KVSD FGLT KEAS STQD TGKL PVKW TAPE ALRE KKFS TKSD VWSF GILL WEIY SFGR VPYP RIPL KDVV PRVE KGYK MDAP DGCP PAVY EVMK NCWH LDAA MRPS FLQL REQL EHIK THEL HL
Gene ID:	1445
Uniprot:	P41240
Cellular Location:	Cell membrane, Cytoplasm
Calculated MW:	50kDa
Observed MW:	50KDa

Synonyms:

CSK

Background:

Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN, CSK or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK.

UniProt Protein Function:	CSK: a tyrosine kinase of the Csk family that phosphorylates and inhibits Src family kinases. Specifically phosphorylates Y504 on LCK, which acts as a negative regulatory site. Csk and the phospho-tyrosine phosphatase CD45 reciprocally regulate phosphorylation of the inhibitory tyrosine of the Src family kinases Lck and Fyn during T-cell receptor mediated signaling during thymic development.
UniProt Protein Details:	Protein type:Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; Protein kinase, TK; Kinase, protein; TK group; Csk family Chromosomal Location of Human Ortholog: 15q24.1 Cellular Component: cytoplasm; cytosol; extrinsic to internal side of plasma membrane; plasma membrane Molecular Function:identical protein binding; kinase activity; non-membrane spanning protein tyrosine kinase activity; protein binding; protein C-terminus binding; protein-tyrosine kinase activity; receptor binding Biological Process: cell differentiation; cell migration; central nervous system development; epidermal growth factor receptor signaling pathway; innate immune response; negative regulation of Golgi to plasma membrane protein transport; protein amino acid phosphorylation; regulation of cell proliferation; regulation of cytokine production; T cell costimulation; T cell receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway
UniProt Code:	P41240
NCBI GenInfo Identifier:	729887
NCBI Gene ID:	1445
NCBI Accession:	P41240.1
UniProt Secondary Accession:	P41240,Q2M3N2, Q6FGZ6,
UniProt Related Accession:	P41240
Molecular Weight:	50,704 Da
NCBI Full Name:	Tyrosine-protein kinase CSK
NCBI Synonym Full Names:	c-src tyrosine kinase
NCBI Official Symbol:	CSK
NCBI Protein Information:	tyrosine-protein kinase CSK
UniProt Protein Name:	Tyrosine-protein kinase CSK
UniProt Synonym Protein Names:	C-Src kinase; Protein-tyrosine kinase CYL
Protein Family:	Chloroplast sensor kinase
UniProt Gene Name:	CSK
UniProt Entry Name:	CSK_HUMAN

	Western blot analysis of extracts from normal (control) and CSK knockout (KO) 293T cells, using CSK antibody (CAB0735) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Western blot analysis of extracts of various cell lines, using CSK antibody (CAB0735) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Western blot analysis of extracts of various cell lines, using CSK antibody (CAB0735) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat spleen using [KO Validated] CSK Rabbit pAb (CAB0735) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] CSK Rabbit pAb (CAB0735) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-CSK Antibody (CAB16824)	Human c-Src / CSK / Tyrosine-protein kinase ELISA Kit
Phospho-CSK (S364) Antibody (PACO02489)	Human Tyrosine-protein kinase CSK (CSK) ELISA Kit
CSK Antibody (PACO02490)	Csk (Phospho-Ser364) Colorimetric Cell-Based ELISA Kit
CSK Antibody (PACO06379)	CSK Colorimetric Cell-Based ELISA Kit
CSK Antibody (PACO08630)