KO Validated Antibodies 1

Anti-CSDE1 Antibody (CAB5941)[KO Validated]

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SKU:
CAB5941
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-CSDE1 Antibody [KO Validated]
Antibody SKU:CAB5941
Antibody Size:20uL, 50uL, 100uL
Application:WB IF IP
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 369-600 of human CSDE1 (NP_009089.4).
Application:WB IF IP
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:20 - 1:50
Reactivity:Human, Mouse
Positive Samples:HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 369-600 of human CSDE1 (NP_009089.4).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LSAQ RNHA IRIK KLPK GTVS FHSH SDHR FLGT VEKE ATFS NPKT TSPN KGKE KEAE DGII AYDD CGVK LTIA FQAK DVEG STSP QIGD KVEF SISD KQRP GQQV ATCV RLLG RNSN SKRL LGYV ATLK DNFG FIET ANHD KEIF FHYS EFSG DVDS LELG DMVE YSLS KGKG NKVS AEKV NKTH SVNG ITEE ADPT IYSG KVIR PLRS VDPT QTEY QGMI EIVE EGDM KGEV
Gene ID:7812
Uniprot:O75534
Cellular Location:Cytoplasm
Calculated MW:85kDa/88kDa/90kDa/93kDa
Observed MW:89KDa
Synonyms:CSDE1, D1S155E, UNR
Background:
UniProt Protein Function:CSDE1: RNA-binding protein. Required for internal initiation of translation of human rhinovirus RNA. May be involved in translationally coupled mRNA turnover. Implicated with other RNA- binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding- region determinant of instability (mCRD) domain. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:RNA-binding; Adaptor/scaffold; Mitochondrial

Chromosomal Location of Human Ortholog: 1p22

Cellular Component: Golgi apparatus; mitochondrial inner membrane; cytoplasm; plasma membrane

Molecular Function:DNA binding

Biological Process: regulation of transcription, DNA-dependent; male gonad development

UniProt Code:O75534
NCBI GenInfo Identifier:12643993
NCBI Gene ID:7812
NCBI Accession:O75534.2
UniProt Secondary Accession:O75534,O94961, Q5TF04, Q5TF05, Q68DF1, Q68DI9, Q9Y2S4 A8K281, E9PGZ0, G5E9Q2,
UniProt Related Accession:O75534
Molecular Weight:798
NCBI Full Name:Cold shock domain-containing protein E1
NCBI Synonym Full Names:cold shock domain containing E1, RNA-binding
NCBI Official Symbol:CSDE1  
NCBI Official Synonym Symbols:UNR; D1S155E  
NCBI Protein Information:cold shock domain-containing protein E1; NRAS-related; upstream of NRAS; N-ras upstream gene protein
UniProt Protein Name:Cold shock domain-containing protein E1
UniProt Synonym Protein Names:N-ras upstream gene protein; Protein UNR
Protein Family:Cold shock domain-containing protein
UniProt Gene Name:CSDE1  
UniProt Entry Name:CSDE1_HUMAN
Anti-CSDE1 Antibody [KO Validated] (CAB5941)Western blot analysis of extracts from normal (control) and CSDE1 knockout (KO) HeLa cells, using CSDE1 antibody (CAB5941) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
Anti-CSDE1 Antibody [KO Validated] (CAB5941)Immunofluorescence analysis of L929 cells using CSDE1 Rabbit pAb (CAB5941) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-CSDE1 Antibody [KO Validated] (CAB5941)Immunoprecipitation analysis of 100ug extracts of HeLa cells using 3ug CSDE1 antibody (CAB5941). Western blot was performed from the immunoprecipitate using CSDE1 antibody (CAB5941) at a dilition of 1:1000.
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