Antibody Name:	Anti-CRYZ Antibody
Antibody SKU:	CAB12857
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-329 of human CRYZ (NP_001123514.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	K-562, Mouse liver, Mouse brain, Mouse kidney, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-329 of human CRYZ (NP_001123514.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MATG QKLM RAVR VFEF GGPE VLKL RSDI AVPI PKDH QVLI KVHA CGVN PVET YIRS GTYS RKPL LPYT PGSD VAGV IEAV GDNA SAFK KGDR VFTS STIS GGYA EYAL AADH TVYK LPEK LDFK QGAA IGIP YFTA YRAL IHSA CVKA GESV LVHG ASGG VGLA ACQI ARAY GLKI LGTA GTEE GQKI VLQN GAHE VFNH REVN YIDK IKKY VGEK GIDI IIEM LANV NLSK DLSL LSHG GRVI VVGS RGTI EINP RDTM AKES SIIG VTLF SSTK EEFQ QYAA ALQA GMEI GWLK PVIG SQYP LEKV AEAH ENII HGSG ATGK MILL L
Gene ID:	1429
Uniprot:	Q08257
Cellular Location:	Cytoplasm
Calculated MW:	20kDa/31kDa/35kDa
Observed MW:	37kDa

Synonyms:

CRYZ

Background:

Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. The former class is also called phylogenetically-restricted crystallins. This gene encodes a taxon-specific crystallin protein which has NADPH-dependent quinone reductase activity distinct from other known quinone reductases. It lacks alcohol dehydrogenase activity although by similarity it is considered a member of the zinc-containing alcohol dehydrogenase family. Unlike other mammalian species, in humans, lens expression is low. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One pseudogene is known to exist.

UniProt Protein Function:	CRYZ: Does not have alcohol dehydrogenase activity. Binds NADP and acts through a one-electron transfer process. Orthoquinones, such as 1,2-naphthoquinone or 9,10-phenanthrenequinone, are the best substrates (in vitro). May act in the detoxification of xenobiotics. Interacts with (AU)-rich elements (ARE) in the 3'-UTR of target mRNA species. Enhances the stability of mRNA coding for BCL2. NADPH binding interferes with mRNA binding. Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 1.6.5.5; Oxidoreductase; RNA-binding Chromosomal Location of Human Ortholog: 1p31.1 Cellular Component: cytoplasm; cytosol Molecular Function:mRNA 3'-UTR binding; NADPH:quinone reductase activity Biological Process: protein homotetramerization; visual perception; xenobiotic catabolic process
NCBI Summary:	Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. The former class is also called phylogenetically-restricted crystallins. This gene encodes a taxon-specific crystallin protein which has NADPH-dependent quinone reductase activity distinct from other known quinone reductases. It lacks alcohol dehydrogenase activity although by similarity it is considered a member of the zinc-containing alcohol dehydrogenase family. Unlike other mammalian species, in humans, lens expression is low. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One pseudogene is known to exist. [provided by RefSeq, Sep 2008]
UniProt Code:	Q08257
NCBI GenInfo Identifier:	585013
NCBI Gene ID:	1429
NCBI Accession:	Q08257.1
UniProt Secondary Accession:	Q08257,Q53FT0, Q59EU7, Q5HYE7, Q6NSK9, A6NN60, D3DQ76
UniProt Related Accession:	Q08257
Molecular Weight:	31,528 Da
NCBI Full Name:	Quinone oxidoreductase
NCBI Synonym Full Names:	crystallin zeta
NCBI Official Symbol:	CRYZ
NCBI Protein Information:	quinone oxidoreductase
UniProt Protein Name:	Quinone oxidoreductase
UniProt Synonym Protein Names:	NADPH:quinone reductase; Zeta-crystallin
Protein Family:	Zeta-crystallin
UniProt Gene Name:	CRYZ
UniProt Entry Name:	QOR_HUMAN

	Western blot analysis of extracts of various cell lines, using CRYZ antibody (CAB12857) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of L929 cells using CRYZ Rabbit pAb (CAB12857) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using CRYZ Rabbit pAb (CAB12857) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.