Cell Biology Antibodies 9

Anti-CRYM Antibody (CAB3810)

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SKU:
CAB3810
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-CRYM Antibody
Antibody SKU:CAB3810
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-314 of human CRYM (NP_001879.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:A-549, HepG2, Jurkat, Mouse brain, Mouse kidney, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-314 of human CRYM (NP_001879.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSRV PAFL SAAE VEEH LRSS SLLI PPLE TALA NFSS GPEG GVMQ PVRT VVPV TKHR GYLG VMPA YSAA EDAL TTKL VTFY EDRG ITSV VPSH QATV LLFE PSNG TLLA VMDG NVIT AKRT AAVS AIAT KFLK PPSS EVLC ILGA GVQA YSHY EIFT EQFS FKEV RIWN RTKE NAEK FADT VQGE VRVC SSVQ EAVA GADV IITV TLAT EPIL FGEW VKPG AHIN AVGA SRPD WREL DDEL MKEA VLYV DSQE AALK ESGD VLLS GAEI FAEL GEVI KGVK PAHC EKTT VFKS LGMA VEDT VAAK LIYD SWSS GK
Gene ID:1428
Uniprot:Q14894
Cellular Location:Cytoplasm
Calculated MW:33kDa
Observed MW:36kDa
Synonyms:CRYM, DFNA40, THBP
Background:Crystallins are separated into two classes: taxon-specific and ubiquitous. The former class is also called phylogenetically-restricted crystallins. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. This gene encodes a taxon-specific crystallin protein that binds NADPH and has sequence similarity to bacterial ornithine cyclodeaminases. The encoded protein does not perform a structural role in lens tissue, and instead it binds thyroid hormone for possible regulatory or developmental roles. Mutations in this gene have been associated with autosomal dominant non-syndromic deafness.
UniProt Protein Function:CRYM: Specifically catalyzes the reduction of imine bonds in brain substrates that may include cystathionine ketimine (CysK) and lanthionine ketimine (LK). Binds thyroid hormone which is a strong reversible inhibitor. Presumably involved in the regulation of the free intracellular concentration of triiodothyronine and access to its nuclear receptors. Belongs to the ornithine cyclodeaminase family.
UniProt Protein Details:

Protein type:Lyase; EC 1.5.1.25

Chromosomal Location of Human Ortholog: 16p12.2

Cellular Component: mitochondrion; cytoplasm; nucleus

Molecular Function:thiomorpholine-carboxylate dehydrogenase activity; protein homodimerization activity; NADP binding; transcription corepressor activity

Biological Process: sensory perception of sound; negative regulation of transcription from RNA polymerase II promoter; thyroid hormone metabolic process

Disease: Deafness, Autosomal Dominant 40

NCBI Summary:Crystallins are separated into two classes: taxon-specific and ubiquitous. The former class is also called phylogenetically-restricted crystallins. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. This gene encodes a taxon-specific crystallin protein that binds NADPH and has sequence similarity to bacterial ornithine cyclodeaminases. The encoded protein does not perform a structural role in lens tissue, and instead it binds thyroid hormone for possible regulatory or developmental roles. Mutations in this gene have been associated with autosomal dominant non-syndromic deafness. [provided by RefSeq, Sep 2014]
UniProt Code:Q14894
NCBI GenInfo Identifier:2498259
NCBI Gene ID:1428
NCBI Accession:Q14894.1
UniProt Related Accession:Q14894
Molecular Weight:36kDa
NCBI Full Name:Ketimine reductase mu-crystallin
NCBI Synonym Full Names:crystallin mu
NCBI Official Symbol:CRYM  
NCBI Official Synonym Symbols:THBP; DFNA40  
NCBI Protein Information:ketimine reductase mu-crystallin
UniProt Protein Name:Ketimine reductase mu-crystallin
UniProt Synonym Protein Names:NADP-regulated thyroid-hormone-binding protein
Protein Family:Ketimine reductase
UniProt Gene Name:CRYM  
UniProt Entry Name:CRYM_HUMAN
Anti-CRYM Antibody (CAB3810)Western blot analysis of extracts of various cell lines, using CRYM antibody (CAB3810) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-CRYM Antibody (CAB3810)Western blot analysis of extracts of rat brain, using CRYM antibody (CAB3810) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit (RM00020)._Exposure time: 30s.
Anti-CRYM Antibody (CAB3810)Immunohistochemistry of paraffin-embedded rat brain using CRYM antibody (CAB3810) at dilution of 1:100 (40x lens).
Anti-CRYM Antibody (CAB3810)Immunohistochemistry of paraffin-embedded mouse brain using CRYM antibody (CAB3810) at dilution of 1:100 (40x lens).
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