Signal Transduction Antibodies 3

Anti-CRYGD Antibody (CAB5740)

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SKU:
CAB5740
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-CRYGD Antibody
Antibody SKU:CAB5740
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 18-117 of human CRYGD (NP_008822.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:BT-474, HepG2, SW620, Raji, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 18-117 of human CRYGD (NP_008822.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ECSS DHPN LQPY LSRC NSAR VDSG CWML YEQP NYSG LQYF LRRG DYAD HQQW MGLS DSVR SCRL IPHS GSHR IRLY ERED YRGQ MIEF TEDC SCLQ DRFR
Gene ID:1421
Uniprot:P07320
Cellular Location:
Calculated MW:20kDa
Observed MW:26kDa
Synonyms:CRYGD, CACA, CCA3, CCP, CRYG4, CTRCT4, PCC, cry-g-D
Background:Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Gamma-crystallins are a homogeneous group of highly symmetrical, monomeric proteins typically lacking connecting peptides and terminal extensions. They are differentially regulated after early development. Four gamma-crystallin genes (gamma-A through gamma-D) and three pseudogenes (gamma-E, gamma-F, gamma-G) are tandemly organized in a genomic segment as a gene cluster. Whether due to aging or mutations in specific genes, gamma-crystallins have been involved in cataract formation.
UniProt Protein Function:CRYGD: a major structural protein of the eye lens. A member of the beta/gamma-crystallin family. Defects are the cause of congenital zonular cataract with sutural opacities. Two splice-variant isoforms have been described.
UniProt Protein Details:

Protein type:Cytoskeletal

Chromosomal Location of Human Ortholog: 2q33.3

Cellular Component: cytoplasm; nucleus

Molecular Function:protein binding

Biological Process: lens development in camera-type eye; visual perception

Disease: Cataract 4, Multiple Types

NCBI Summary:Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Gamma-crystallins are a homogeneous group of highly symmetrical, monomeric proteins typically lacking connecting peptides and terminal extensions. They are differentially regulated after early development. Four gamma-crystallin genes (gamma-A through gamma-D) and three pseudogenes (gamma-E, gamma-F, gamma-G) are tandemly organized in a genomic segment as a gene cluster. Whether due to aging or mutations in specific genes, gamma-crystallins have been involved in cataract formation. [provided by RefSeq, Jul 2008]
UniProt Code:P07320
NCBI GenInfo Identifier:2506321
NCBI Gene ID:1421
NCBI Accession:P07320.3
UniProt Secondary Accession:P07320,Q17RF7, Q53R51, Q99681,
UniProt Related Accession:P07320
Molecular Weight:20,738 Da
NCBI Full Name:Gamma-crystallin D
NCBI Synonym Full Names:crystallin gamma D
NCBI Official Symbol:CRYGD  
NCBI Official Synonym Symbols:CCP; PCC; CACA; CCA3; CRYG4; CTRCT4; cry-g-D  
NCBI Protein Information:gamma-crystallin D
UniProt Protein Name:Gamma-crystallin D
UniProt Synonym Protein Names:Gamma-D-crystallin; Gamma-crystallin 4
Protein Family:Gamma-crystallin
UniProt Gene Name:CRYGD  
UniProt Entry Name:CRGD_HUMAN
Anti-CRYGD Antibody (CAB5740)Western blot analysis of extracts of various cell lines, using CRYGD antibody (CAB5740) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
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