Name: Anti-CRYBB1 Antibody (CAB2590)
Brand: Cell Biology Antibodies 8
SKU: CAB2590
Availability: OutOfStock

Antibody Name:	Anti-CRYBB1 Antibody
Antibody SKU:	CAB2590
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-252 of human CRYBB1 (NP_001878.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	MCF7, BT474

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-252 of human CRYBB1 (NP_001878.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSQA AKAS ASAT VAVN PGPD TKGK GAPP AGTS PSPG TTLA PTTV PITS AKAA ELPP GNYR LVVF ELEN FQGR RAEF SGEC SNLA DRGF DRVR SIIV SAGP WVAF EQSN FRGE MFIL EKGE YPRW NTWS SSYR SDRL MSFR PIKM DAQE HKIS LFEG ANFK GNTI EIQG DDAP SLWV YGFS DRVG SVKV SSGT WVGY QYPG YRGY QYLL EPGD FRHW NEWG AFQP QMQS LRRL RDKQ WHLE GSFP VLAT EPPK
Gene ID:	1414
Uniprot:	P53674
Cellular Location:
Calculated MW:	28kDa
Observed MW:	36kDa

Synonyms:

CRYBB1, CATCN3, CTRCT17

Background:

Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene, a beta basic group member, undergoes extensive cleavage at its N-terminal extension during lens maturation. It is also a member of a gene cluster with beta-A4, beta-B2, and beta-B3.

UniProt Protein Function:	CRYBB1: a major structural protein of the eye lens. A member of the beta/gamma-crystallin family. Specific cleavages in the N-terminal arm occur during lens maturation and give rise to truncated forms, leading to impaired oligomerization and protein insolubilization. The protease responsible for this partial degradation could be calpain II.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 22q12.1 Molecular Function:structural constituent of eye lens Biological Process: visual perception Disease: Cataract 17, Multiple Types
NCBI Summary:	Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene, a beta basic group member, undergoes extensive cleavage at its N-terminal extension during lens maturation. It is also a member of a gene cluster with beta-A4, beta-B2, and beta-B3. [provided by RefSeq, Jul 2008]
UniProt Code:	P53674
NCBI GenInfo Identifier:	1706116
NCBI Gene ID:	1414
NCBI Accession:	P53674.2
UniProt Secondary Accession:	P53674,Q9WVJ5,
UniProt Related Accession:	P53674
Molecular Weight:	252
NCBI Full Name:	Beta-crystallin B1
NCBI Synonym Full Names:	crystallin, beta B1
NCBI Official Symbol:	CRYBB1
NCBI Official Synonym Symbols:	CATCN3; CTRCT17
NCBI Protein Information:	beta-crystallin B1; beta-B1 crystallin; eye lens structural protein
UniProt Protein Name:	Beta-crystallin B1
UniProt Synonym Protein Names:	Beta-B1 crystallin
Protein Family:	Beta-crystallin
UniProt Gene Name:	CRYBB1
UniProt Entry Name:	CRBB1_HUMAN

Western blot analysis of extracts of various cell lines, using CRYBB1 antibody (CAB2590) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021).

Antibodies

CRYBB1 Antibody (PACO08616)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)