Cell Biology Antibodies 10

Anti-CRY2 Antibody (CAB6891)

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SKU:
CAB6891
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-CRY2 Antibody
Antibody SKU:CAB6891
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 455-614 of human CRY2 (NP_066940.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Rat
Positive Samples:HepG2, A-549, Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 455-614 of human CRY2 (NP_066940.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PVGF GRRT DPSG DYIR RYLP KLKA FPSR YIYE PWNA PESI QKAA KCII GVDY PRPI VNHA ETSR LNIE RMKQ IYQQ LSRY RGLC LLAS VPSC VEDL SHPV AEPS SSQA GSMS SAGP RPLP SGPA SPKR KLEA AEEP PGEE LSKR ARVA ELPT PELP SKDA
Gene ID:1408
Uniprot:Q49AN0
Cellular Location:Cytoplasm, Nucleus
Calculated MW:60kDa/66kDa
Observed MW:67KDa
Synonyms:CRY2, HCRY2, PHLL2
Background:This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. Two transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:CRY2: Blue light-dependent regulator of the circadian feedback loop. Inhibits CLOCK|NPAS2-ARNTL E box-mediated transcription. Acts, in conjunction with CRY2, in maintaining period length and circadian rhythmicity. Has no photolyase activity. Capable of translocating circadian clock core proteins such as PER proteins to the nucleus. May inhibit CLOCK|NPAS2-ARNTL transcriptional activity through stabilizing the unphosphorylated form of ARNTL. Belongs to the DNA photolyase class-1 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:DNA-binding; Lyase

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: cytoplasm; extracellular region; nucleus

Molecular Function:ubiquitin binding; protein binding; DNA binding; DNA (6-4) photolyase activity; damaged DNA binding; deoxyribodipyrimidine photo-lyase activity; blue light photoreceptor activity; single-stranded DNA binding; phosphatase binding

Biological Process: circadian rhythm; photoreactive repair; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; glucose homeostasis; regulation of circadian rhythm; protein-chromophore linkage; entrainment of circadian clock by photoperiod; blue light signaling pathway; negative regulation of phosphoprotein phosphatase activity; negative regulation of circadian rhythm; circadian regulation of gene expression; negative regulation of transcription, DNA-dependent

NCBI Summary:This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2014]
UniProt Code:Q49AN0
NCBI GenInfo Identifier:118572252
NCBI Gene ID:1408
NCBI Accession:Q49AN0.2
UniProt Secondary Accession:Q49AN0,O75148, Q8IV71, B4DH32, B4DZD6,
UniProt Related Accession:Q49AN0
Molecular Weight:
NCBI Full Name:Cryptochrome-2
NCBI Synonym Full Names:cryptochrome circadian clock 2
NCBI Official Symbol:CRY2  
NCBI Official Synonym Symbols:HCRY2; PHLL2  
NCBI Protein Information:cryptochrome-2; growth-inhibiting protein 37; cryptochrome 2 (photolyase-like)
UniProt Protein Name:Cryptochrome-2
Protein Family:Cryptochrome
UniProt Gene Name:CRY2  
UniProt Entry Name:CRY2_HUMAN
Anti-CRY2 Antibody (CAB6891)Western blot analysis of extracts of various cell lines, using CRY2 antibody (CAB6891) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s.
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