Name: Anti-CRY1 Antibody (CAB18028)[KO Validated]
Brand: KO Validated Antibodies 1
SKU: CAB18028
Availability: OutOfStock

Antibody Name:	Anti-CRY1 Antibody [KO Validated]
Antibody SKU:	CAB18028
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 507-586 of human CRY1 (NP_004066.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 507-586 of human CRY1 (NP_004066.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PNGN GGFM GYSA ENIP GCSS SGSC SQGS GILH YAHG DSQQ THLL KQGR SSMG TGLS GGKR PSQE EDTQ SIGP KVQR QSTN
Gene ID:	1407
Uniprot:	Q16526
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	66kDa
Observed MW:	65kDa

Anti-CRY1 Antibody [KO Validated] (CAB18028)

Western blot analysis of extracts from normal (control) and CRY1 knockout (KO) HeLa cells, using CRY1 antibody (CAB18028) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Synonyms:

CRY1, PHLL1, DSPD

Background:

This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. Loss of the related gene in mouse results in a shortened circadian cycle in complete darkness.

UniProt Protein Function:	CRY1: Blue light-dependent regulator of the circadian feedback loop. Inhibits CLOCK\|NPAS2-ARNTL E box-mediated transcription. Acts, in conjunction with CRY2, in maintaining period length and circadian rhythmicity. Has no photolyase activity. Capable of translocating circadian clock core proteins such as PER proteins to the nucleus. May inhibit CLOCK\|NPAS2-ARNTL transcriptional activity through stabilizing the unphosphorylated form of ARNTL. Component of the circadian core oscillator, which includes the CRY proteins, CLOCK or NPAS2, ARNTL or ARNTL2, CSNK1D and/or CSNK1E, TIMELESS, and the PER proteins. Interacts directly with TIMELESS and the PER proteins. Interacts directly with PER1 and PER2 C-terminal domains. Interaction with PER2 inhibits its ubiquitination and vice versa. Binds MAPK. Interacts with FBXL21. Interacts with FBXL3. Expression is regulated by light and circadian rhythms. Peak expression in the suprachiasma nucleus (SCN) and eye at the day/night transition (CT12). Levels decrease with ARNTL-CLOCK inhibition as part of the autoregulatory feedback loop. Belongs to the DNA photolyase class-1 family.
UniProt Protein Details:	Protein type:DNA-binding; Transcription, coactivator/corepressor; Lyase Chromosomal Location of Human Ortholog: 12q23-q24.1 Cellular Component: mitochondrion; nucleus Molecular Function:ubiquitin binding; histone deacetylase binding; DNA (6-4) photolyase activity; nucleotide binding; transcription factor binding; protein kinase binding; phosphatase binding; protein binding; DNA binding; double-stranded DNA binding; deoxyribodipyrimidine photo-lyase activity; blue light photoreceptor activity; nuclear hormone receptor binding Biological Process: circadian rhythm; response to glucagon stimulus; transcription, DNA-dependent; DNA damage induced protein phosphorylation; negative regulation of G-protein coupled receptor protein signaling pathway; negative regulation of transcription from RNA polymerase II promoter; glucose homeostasis; DNA repair; regulation of circadian rhythm; protein-chromophore linkage; response to insulin stimulus; gluconeogenesis; entrainment of circadian clock by photoperiod; blue light signaling pathway; sequestering of lipid; negative regulation of circadian rhythm; negative regulation of protein ubiquitination; circadian regulation of gene expression; negative regulation of transcription, DNA-dependent
NCBI Summary:	This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. Loss of the related gene in mouse results in a shortened circadian cycle in complete darkness. [provided by RefSeq, Jan 2014]
UniProt Code:	Q16526
NCBI GenInfo Identifier:	74735764
NCBI Gene ID:	1407
NCBI Accession:	Q16526.1
UniProt Related Accession:	Q16526
Molecular Weight:	586
NCBI Full Name:	Cryptochrome-1
NCBI Synonym Full Names:	cryptochrome circadian clock 1
NCBI Official Symbol:	CRY1
NCBI Official Synonym Symbols:	PHLL1
NCBI Protein Information:	cryptochrome-1; cryptochrome 1 (photolyase-like)
UniProt Protein Name:	Cryptochrome-1
Protein Family:	Cryptochrome
UniProt Gene Name:	CRY1
UniProt Entry Name:	CRY1_HUMAN

Antibodies	ELISA Kits
Anti-CRY1 Antibody (CAB13662)	Human CRY1 / Cryptochrome-1 ELISA Kit
Anti-CRY1 Antibody (CAB13666)	Human CRY1 (Cryptochrome 1) CLIA Kit (HUES01181)
Anti-CRY1 Antibody (CAB6890)
CRY1 Antibody (PACO08613)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)