Immunology Antibodies 2

Anti-CRTC3 Antibody (CAB15890)

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SKU:
CAB15890
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-CRTC3 Antibody
Antibody SKU:CAB15890
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human CRTC3 (NP_073606.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human CRTC3 (NP_073606.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAAS PGSG SANP RKFS EKIA LHTQ RQAE ETRA FEQL MTDL TLSR VQFQ KLQQ LRLT QYHG GSLP NVSQ LRSS ASEF QPSF HQAD NVRG TRHH GLVE RPSR NRFH PLHR RSGD KPGR QFDG SAFG ANYS SQPL DESW PRQQ PPWK DEKH PGFR LTSA LNRT NSDS ALHT SALS TKPQ DPYG GGGQ SAWP APYM GFCD GENN GHGE VASF PGPL KEEN LLNV PKPL PKQL WETK EIQS LSGR PRSC DVGG GNAF PHNG QNLG
Gene ID:64784
Uniprot:Q6UUV7
Cellular Location:Cytoplasm, Nucleus
Calculated MW:66kDa
Observed MW:75kDa
Synonyms:CRTC3, TORC-3, TORC3
Background:This gene is a member of the CREB regulated transcription coactivator gene family. This family regulates CREB-dependent gene transcription in a phosphorylation-independent manner and may be selective for cAMP-responsive genes. The protein encoded by this gene may induce mitochondrial biogenesis and attenuate catecholamine signaling in adipose tissue. A translocation event between this gene and Notch coactivator mastermind-like gene 2, which results in a fusion protein, has been reported in mucoepidermoid carcinomas. Alternative splicing results in multiple transcript variants that encode different protein isoforms.
UniProt Protein Function:TORC3: Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR). Binding, as a tetramer, through its N-terminal region, with the bZIP domain of CREB1 enhances recruitment of TAF4 to the promoter. 'Arg-314' in the bZIP domain of CREB1 is essential for this interaction. Interaction with HTLV-1 TAX enhances its transcriptional activity. Interacts, via the N- terminal with the ankyrin repeats of BCL3, to form a complex with CREB1 on CRE and TxRE responsive elements and represses HTLV-1 LTR-mediated transcription. Predominantly expressed in B and T lymphocytes. Highest levels in lung. Also expressed in brain, colon, heart, kidney, ovary, and prostate. Weak expression in liver, pancreas, muscle, small intestine, spleen and stomach. Belongs to the TORC family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 15q26.1

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function:cAMP response element binding protein binding

Biological Process: activation of CREB transcription factor; macrophage activation; positive regulation of transcription from RNA polymerase II promoter

NCBI Summary:This gene is a member of the CREB regulated transcription coactivator gene family. This family regulates CREB-dependent gene transcription in a phosphorylation-independent manner and may be selective for cAMP-responsive genes. The protein encoded by this gene may induce mitochondrial biogenesis and attenuate catecholamine signaling in adipose tissue. A translocation event between this gene and Notch coactivator mastermind-like gene 2, which results in a fusion protein, has been reported in mucoepidermoid carcinomas. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jul 2012]
UniProt Code:Q6UUV7
NCBI GenInfo Identifier:167009130
NCBI Gene ID:64784
NCBI Accession:Q6UUV7.2
UniProt Secondary Accession:Q6UUV7,Q6DK61, Q6DK62, Q8NF38, Q9H6U2,
UniProt Related Accession:Q6UUV7
Molecular Weight:66,830 Da
NCBI Full Name:CREB-regulated transcription coactivator 3
NCBI Synonym Full Names:CREB regulated transcription coactivator 3
NCBI Official Symbol:CRTC3  
NCBI Official Synonym Symbols:TORC3; TORC-3  
NCBI Protein Information:CREB-regulated transcription coactivator 3
UniProt Protein Name:CREB-regulated transcription coactivator 3
UniProt Synonym Protein Names:Transducer of regulated cAMP response element-binding protein 3; TORC-3; Transducer of CREB protein 3
Protein Family:CREB-regulated transcription coactivator
UniProt Gene Name:CRTC3  
Anti-CRTC3 Antibody (CAB15890)Western blot analysis of extracts of 293T cells, using CRTC3 antibody (CAB15890) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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