Name: Anti-CRTC1 Antibody (CAB15412)
Brand: Cell Biology Antibodies 6
SKU: CAB15412
Availability: OutOfStock

Antibody Name:	Anti-CRTC1 Antibody
Antibody SKU:	CAB15412
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human CRTC1 (NP_056136.2).

Application:	WB
Recommended Dilution:	WB 1:200 - 1:2000
Reactivity:	Mouse, Rat
Positive Samples:	Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human CRTC1 (NP_056136.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MATS NNPR KFSE KIAL HNQK QAEE TAAF EEVM KDLS LTRA ARLQ LQKS QYLQ LGPS RGQY YGGS LPNV NQIG SGTM DLPF QTPF QSSG LDTS RTTR HHGL VDRV YRER GRLG SPHR RPLS VDKH GRQA DSCP YGTM YLSP PADT SWRR TNSD SALH QSTM TPTQ PESF SSGS QDVH QKRV LLLT VPGM EETT SEAD KNLS
Gene ID:	23373
Uniprot:	Q6UUV9
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	62kDa/67kDa/68kDa
Observed MW:	80kDa

Synonyms:	CRTC1, MECT1, TORC-1, TORC1, WAMTP1
Background:

UniProt Protein Function:	TORC1: Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR). In the hippocampus, involved in late-phase long- term potentiation (L-LTP) maintenance at the Schaffer collateral- CA1 synapses. May be required for dendritic growth of developing cortical neurons. Binds, as a tetramer, through its N-terminal region, with the bZIP domain of CREB1. 'Arg-314' in the bZIP domain of CREB1 is essential for this interaction. Interaction, via its C-terminal, with TAF4, enhances recruitment of TAF4 to CREB1. Binds HTLV1 Tax. Highly expressed in adult and fetal brain. Located to specific regions such as the prefrontal cortex and cerebellum. Very low expression in other tissues such as heart, spleen, lung, skeletal muscle, salivary gland, ovary and kidney. Belongs to the TORC family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transcription factor Chromosomal Location of Human Ortholog: 19p13.11 Cellular Component: nucleoplasm; cytoplasm; plasma membrane; nucleus Molecular Function:protein binding; cAMP response element binding protein binding Biological Process: viral reproduction; transcription, DNA-dependent; membrane hyperpolarization; rhythmic process; activation of CREB transcription factor; positive regulation of transcription from RNA polymerase II promoter; memory; protein homotetramerization; entrainment of circadian clock by photoperiod
UniProt Code:	Q6UUV9
NCBI GenInfo Identifier:	68565585
NCBI Gene ID:	23373
NCBI Accession:	Q6UUV9.2
UniProt Related Accession:	Q6UUV9
Molecular Weight:	62kDa; 67kDa; 68kDa
NCBI Full Name:	CREB-regulated transcription coactivator 1
NCBI Synonym Full Names:	CREB regulated transcription coactivator 1
NCBI Official Symbol:	CRTC1
NCBI Official Synonym Symbols:	MAML2; MECT1; Mam-2; TORC1; TORC-1; WAMTP1
NCBI Protein Information:	CREB-regulated transcription coactivator 1
UniProt Protein Name:	CREB-regulated transcription coactivator 1
UniProt Synonym Protein Names:	Mucoepidermoid carcinoma translocated protein 1; Transducer of regulated cAMP response element-binding protein 1; TORC-1; Transducer of CREB protein 1
Protein Family:	CREB-regulated transcription coactivator
UniProt Gene Name:	CRTC1
UniProt Entry Name:	CRTC1_HUMAN

Western blot analysis of extracts of Mouse brain, using CRTC1 Rabbit pAb (CAB15412) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.

Antibodies

CRTC1 Antibody (PACO08611)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)