Immunology Antibodies 1

Anti-CRP Antibody (CAB0224)

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SKU:
CAB0224
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-CRP Antibody
Antibody SKU:CAB0224
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-224 of human CRP (NP_000558.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Human serum, Mouse liver, Mouse lung, Rat spleen
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-224 of human CRP (NP_000558.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MEKL LCFL VLTS LSHA FGQT DMSR KAFV FPKE SDTS YVSL KAPL TKPL KAFT VCLH FYTE LSST RGYS IFSY ATKR QDNE ILIF WSKD IGYS FTVG GSEI LFEV PEVT VAPV HICT SWES ASGI VEFW VDGK PRVR KSLK KGYT VGAE ASII LGQE QDSF GGNF EGSQ SLVG DIGN VNMW DFVL SPDE INTI YLGG PFSP NVLN WRAL KYEV QGEV FTKP QLWP
Gene ID:1401
Uniprot:P02741
Cellular Location:Secreted
Calculated MW:10kDa/25kDa
Observed MW:25kDa
Synonyms:CRP, PTX1
Background:The protein encoded by this gene belongs to the pentaxin family. It is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host and to initiate their elimination by interacting with humoral and cellular effector systems in the blood. Consequently, the level of this protein in plasma increases greatly during acute phase response to tissue injury, infection, or other inflammatory stimuli.
UniProt Protein Function:Function: Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
UniProt Protein Details:

Cofactor: Binds 2 calcium ions per subunit.

Subunit structure: Homopentamer. Pentaxin (or pentraxin) have a discoid arrangement of 5 non-covalently bound subunits.

Subcellular location: Secreted.

Tissue specificity: Found in plasma.

Induction: The concentration of CRP in plasma increases greatly during acute phase response to tissue injury, infection or other inflammatory stimuli. It is induced by IL1/interleukin-1 and IL6//interleukin-6.

Miscellaneous: This protein owes its name to its ability precipitate pneumococcal C-polysaccharide in the presence of calcium.

Sequence similarities: Belongs to the pentaxin family.Contains 1 pentaxin domain.

Mass spectrometry: Molecular mass is 23028 Da from positions 19 - 224. Determined by MALDI. Ref.15Molecular mass is 22930 Da from positions 19 - 223. Determined by MALDI. Ref.15

NCBI Summary:The protein encoded by this gene belongs to the pentaxin family. It is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host and to initiate their elimination by interacting with humoral and cellular effector systems in the blood. Consequently, the level of this protein in plasma increases greatly during acute phase response to tissue injury, infection, or other inflammatory stimuli. [provided by RefSeq]
UniProt Code:P02741
NCBI GenInfo Identifier:117486
NCBI Gene ID:1401
NCBI Accession:P02741.1
UniProt Secondary Accession:P02741,Q08AK3, Q8WW75, A8K078, D3DVD9, D3DVE0,
UniProt Related Accession:P02741,Q5VVP7
Molecular Weight:25,039 Da
NCBI Full Name:C-reactive protein
NCBI Synonym Full Names:C-reactive protein, pentraxin-related
NCBI Official Symbol:CRP  
NCBI Official Synonym Symbols:PTX1; MGC88244; MGC149895  
NCBI Protein Information:C-reactive protein; pentraxin 1; OTTHUMP00000033307
UniProt Protein Name:C-reactive protein
Protein Family:Cryparin
UniProt Gene Name:CRP  
UniProt Entry Name:CRP_HUMAN
Anti-CRP Antibody (CAB0224)Western blot analysis of extracts of various cell lines, using CRP antibody (CAB0224) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-CRP Antibody (CAB0224)Immunofluorescence analysis of L929 cells using CRP Rabbit pAb (CAB0224) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-CRP Antibody (CAB0224)Immunofluorescence analysis of U2OS cells using CRP Rabbit pAb (CAB0224) at dilution of 1:100. Blue: DAPI for nuclear staining.
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