KO Validated Antibodies 1

Anti-CRKL Antibody (CAB11735)[KO Validated]

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SKU:
CAB11735
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-CRKL Antibody [KO Validated]
Antibody SKU:CAB11735
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-303 of human CRKL (NP_005198.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:LO2, Jurkat, SKOV3, Mouse brain, Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-303 of human CRKL (NP_005198.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSSA RFDS SDRS AWYM GPVS RQEA QTRL QGQR HGMF LVRD SSTC PGDY VLSV SENS RVSH YIIN SLPN RRFK IGDQ EFDH LPAL LEFY KIHY LDTT TLIE PAPR YPSP PMGS VSAP NLPT AEDN LEYV RTLY DFPG NDAE DLPF KKGE ILVI IEKP EEQW WSAR NKDG RVGM IPVP YVEK LVRS SPHG KHGN RNSN SYGI PEPA HAYA QPQT TTPL PAVS GSPG AAIT PLPS TQNG PVFA KAIQ KRVP CAYD KTAL ALEV GDIV KVTR MNIN GQWE GEVN GRKG LFPF THVK IFDP QNPD ENE
Gene ID:1399
Uniprot:P46109
Cellular Location:
Calculated MW:33kDa
Observed MW:37kDa
Synonyms:CRKL
Background:This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.
UniProt Protein Function:CRKL: an oncogenic adaptor protein with an SH2-SH3-SH3 domain structure. Plays a specific role in integrin-induced migration as a downstream mediator of Src. Activates the RAS and Jnk signaling pathways. Transforms fibroblasts in a RAS-dependent fashion. A substrate of BCR-ABL and plays a role in fibroblast transformation by BCR-ABL.
UniProt Protein Details:

Protein type:Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: cytosol; endosome

Molecular Function:protein binding; SH3/SH2 adaptor activity; signal transducer activity

Biological Process: activation of MAPKK activity; JNK cascade; positive regulation of cell proliferation; Ras protein signal transduction

NCBI Summary:This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.[provided by RefSeq, Jan 2009]
UniProt Code:P46109
NCBI GenInfo Identifier:1169094
NCBI Gene ID:1399
NCBI Accession:P46109.1
UniProt Secondary Accession:P46109,A8KA44, D3DX35,
UniProt Related Accession:P46109
Molecular Weight:33,777 Da
NCBI Full Name:Crk-like protein
NCBI Synonym Full Names:CRK like proto-oncogene, adaptor protein
NCBI Official Symbol:CRKL  
NCBI Protein Information:crk-like protein
UniProt Protein Name:Crk-like protein
Protein Family:Crk-like protein
UniProt Gene Name:CRKL  
UniProt Entry Name:CRKL_HUMAN
Anti-CRKL Antibody [KO Validated] (CAB11735)Western blot analysis of extracts of various cell lines, using CRKL Antibody (CAB11735) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-CRKL Antibody [KO Validated] (CAB11735)Western blot analysis of extracts from normal (control) and CRKL knockout (KO) HeLa cells, using CRKL antibody (CAB11735) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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