Cell Biology Antibodies 12

Anti-CRHR1 Antibody (CAB8409)

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SKU:
CAB8409
Product Type:
Antibody
Applications:
WB
Applications:
IHC
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-CRHR1 Antibody
Antibody SKU:CAB8409
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 24-120 of human CRHR1 (NP_001138618.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, SH-SY5Y, HT-29, HeLa, Mouse brain, Mouse liver, Mouse testis, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 24-120 of human CRHR1 (NP_001138618.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SLQD QHCE SLSL ASNI SGLQ CNAS VDLI GTCW PRSP AGQL VVRP CPAF FYGV RYNT TNNG YREC LANG SWAA RVNY SECQ EILN EEKK SKVH YHVA V
Gene ID:1394
Uniprot:P34998
Cellular Location:Cell membrane, Endosome, Multi-pass membrane protein
Calculated MW:28kDa/43kDa/46kDa/47kDa/50kDa
Observed MW:60kDa
Synonyms:CRHR1, CRF-R, CRF-R-1, CRF-R1, CRF1, CRFR-1, CRFR1, CRH-R-1, CRH-R1, CRHR, CRHR1L
Background:This gene encodes a G-protein coupled receptor that binds neuropeptides of the corticotropin releasing hormone family that are major regulators of the hypothalamic-pituitary-adrenal pathway. The encoded protein is essential for the activation of signal transduction pathways that regulate diverse physiological processes including stress, reproduction, immune response and obesity. Alternative splicing results in multiple transcript variants. Naturally-occurring readthrough transcription between this gene and upstream GeneID:147081 results in transcripts that encode isoforms that share similarity with the products of this gene.
UniProt Protein Function:CRF-R1: a G-protein coupled receptor that specifically binds corticotropin-releasing hormone, a potent mediator of endocrine, autonomic, behavioral, and immune responses to stress. Four splice-variant isoforms have been described.
UniProt Protein Details:

Protein type:Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 2

Chromosomal Location of Human Ortholog: 17q21.31

Cellular Component: integral to membrane; integral to plasma membrane; intrinsic to plasma membrane; plasma membrane

Molecular Function:corticotrophin-releasing factor receptor activity; protein binding

Biological Process: adenylate cyclase activation; adrenocorticotropin hormone secretion; immune response; parturition

NCBI Summary:This locus represents naturally occurring readthrough transcription between neighboring GeneID:401884 (ADP-ribosylation factor 3 pseudogene) and GeneID:1394 (CRHR1, corticotropin releasing hormone receptor 1) on chromosome 17. The readthrough transcript encodes a protein that shares sequence identity with the product of the CRHR1 gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
UniProt Code:P34998
NCBI GenInfo Identifier:461836
NCBI Gene ID:104909134
NCBI Accession:P34998.1
UniProt Secondary Accession:P34998,Q13008, Q4QRJ1, Q9UK64, B4DIE9,
Molecular Weight:28,122 Da
NCBI Full Name:Corticotropin-releasing factor receptor 1
NCBI Official Symbol:MGC57346-CRHR1  
NCBI Protein Information:MGC57346-CRHR1 protein
UniProt Protein Name:Corticotropin-releasing factor receptor 1
UniProt Synonym Protein Names:Corticotropin-releasing hormone receptor 1; CRH-R-1; CRH-R1
Protein Family:Corticotropin-releasing factor receptor
UniProt Gene Name:CRHR1  
UniProt Entry Name:CRFR1_HUMAN
Anti-CRHR1 Polyclonal Antibody (CAB8409)Western blot analysis of extracts of various cell lines, using CRHR1 antibody (CAB8409) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s.
Anti-CRHR1 Polyclonal Antibody (CAB8409)Immunohistochemistry of paraffin-embedded rat brain using CRHR1 antibody (CAB8409) at dilution of 1:100 (40x lens).
Anti-CRHR1 Polyclonal Antibody (CAB8409)Immunohistochemistry of paraffin-embedded mouse brain using CRHR1 antibody (CAB8409) at dilution of 1:100 (40x lens).
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