Metabolism Antibodies 2
Anti-CRAT Antibody (CAB6365)
- SKU:
- CAB6365
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-CRAT Antibody |
| Antibody SKU: | CAB6365 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 357-626 of human CRAT (NP_000746.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse |
| Positive Samples: | Mouse skeletal muscle, Mouse heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 357-626 of human CRAT (NP_000746.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | YVIE YTKK PELV RSPM VPLP MPKK LRFN ITPE IKSD IEKA KQNL SIMI QDLD ITVM VFHH FGKD FPKS EKLS PDAF IQMA LQLA YYRI YGQA CATY ESAS LRMF HLGR TDTI RSAS MDSL TFVK AMDD SSVT EHQK VELL RKAV QAHR GYTD RAIR GEAF DRHL LGLK LQAI EDLV SMPD IFMD TSYA IAMH FHLS TSQV PAKT DCVM FFGP VVPD GYGV CYNP MEAH INFS LSAY NSCA ETNA ARLA HYLE KALL DMRA LLQS HPRA KL |
| Gene ID: | 1384 |
| Uniprot: | P43155 |
| Cellular Location: | Endoplasmic reticulum, Matrix side, Mitochondrion, Mitochondrion inner membrane, Peripheral membrane protein, Peroxisome |
| Calculated MW: | 61kDa/68kDa/70kDa |
| Observed MW: | 60kDa |
| Synonyms: | CRAT, CAT, CAT1 |
| Background: | This gene encodes carnitine acetyltransferase (CRAT), which is a key enzyme in the metabolic pathway in mitochondria, peroxisomes and endoplasmic reticulum. CRAT catalyzes the reversible transfer of acyl groups from an acyl-CoA thioester to carnitine and regulates the ratio of acylCoA/CoA in the subcellular compartments. Two transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | CRAT: Carnitine acetylase is specific for short chain fatty acids. Carnitine acetylase seems to affect the flux through the pyruvate dehydrogenase complex. It may be involved as well in the transport of acetyl-CoA into mitochondria. Belongs to the carnitine/choline acetyltransferase family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 2.3.1.7; Mitochondrial; Acetyltransferase Chromosomal Location of Human Ortholog: 9q34.1 Cellular Component: peroxisomal matrix; mitochondrion; endoplasmic reticulum; mitochondrial inner membrane; peroxisome Molecular Function:carnitine O-acetyltransferase activity; receptor binding Biological Process: carnitine metabolic process, CoA-linked; fatty acid beta-oxidation using acyl-CoA oxidase; transport; cellular lipid metabolic process |
| NCBI Summary: | This gene encodes carnitine acetyltransferase (CRAT), which is a key enzyme in the metabolic pathway in mitochondria, peroxisomes and endoplasmic reticulum. CRAT catalyzes the reversible transfer of acyl groups from an acyl-CoA thioester to carnitine and regulates the ratio of acylCoA/CoA in the subcellular compartments. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012] |
| UniProt Code: | P43155 |
| NCBI GenInfo Identifier: | 215274265 |
| NCBI Gene ID: | 1384 |
| NCBI Accession: | P43155.5 |
| UniProt Secondary Accession: | P43155,Q5T952, Q9BW16, |
| UniProt Related Accession: | P43155 |
| Molecular Weight: | 626 |
| NCBI Full Name: | Carnitine O-acetyltransferase |
| NCBI Synonym Full Names: | carnitine O-acetyltransferase |
| NCBI Official Symbol: | CRAT |
| NCBI Official Synonym Symbols: | CAT1 |
| NCBI Protein Information: | carnitine O-acetyltransferase; CAT; carnitine acetylase; carnitine acetyltransferase |
| UniProt Protein Name: | Carnitine O-acetyltransferase |
| UniProt Synonym Protein Names: | Carnitine acetyltransferase; CAT; CrAT |
| Protein Family: | Carnitine O-acetyltransferase |
| UniProt Gene Name: | CRAT |
| UniProt Entry Name: | CACP_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CRAT antibody (CAB6365) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Immunofluorescence analysis of HeLa cells using CRAT antibody (CAB6365). Blue: DAPI for nuclear staining. |
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