Name: Anti-CRADD Antibody (CAB1124)
Brand: Cell Death Antibodies 1
SKU: CAB1124
Availability: OutOfStock

Antibody Name:	Anti-CRADD Antibody
Antibody SKU:	CAB1124
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-199 of human CRADD (NP_003796.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	HepG2, 293T, K-562

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-199 of human CRADD (NP_003796.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEAR DKQV LRSL RLEL GAEV LVEG LVLQ YLYQ EGIL TENH IQEI NAQT TGLR KTML LLDI LPSR GPKA FDTF LDSL QEFP WVRE KLKK AREE AMTD LPAG DRLT GIPS HILN SSPS DRQI NQLA QRLG PEWE PMVL SLGL SQTD IYRC KANH PHNV QSQV VEAF IRWR QRFG KQAT FQSL HNGL RAVE VDPS LLLH MLE
Gene ID:	8738
Uniprot:	P78560
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	11kDa/22kDa
Observed MW:	22kDa

Synonyms:

CRADD, MRT34, RAIDD

Background:

This gene encodes a protein containing a death domain (DD) motif. This protein recruits caspase 2/ICH1 to the cell death signal transduction complex, which includes tumor necrosis factor receptor 1 (TNFR1A) and RIPK1/RIP kinase, and acts in promoting apoptosis. A mutation in this gene was associated with mental retardation. A related pseudogene is found on chromosome 3. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	CRADD: Apoptotic adaptor molecule specific for caspase-2 and FASL/TNF receptor-interacting protein RIP. In the presence of RIP and TRADD, CRADD recruits caspase-2 to the TNFR-1 signalling complex. Defects in CRADD are the cause of mental retardation autosomal recessive type 34 (MRT34). A disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRT34 is a non- syndromic form. Affected individuals have mildly delayed development and significantly impaired cognitive function, precluding independent living and self-care. Speech is rudimentary, but articulate; autism is not present.
UniProt Protein Details:	Protein type:Adaptor/scaffold; Apoptosis Chromosomal Location of Human Ortholog: 12q21.33-q23.1 Cellular Component: cytoplasm; nucleus Molecular Function:protein binding, bridging; protein binding; protease binding Biological Process: caspase activation; induction of apoptosis via death domain receptors; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest Disease: Mental Retardation, Autosomal Recessive 34
NCBI Summary:	This gene encodes a protein containing a death domain (DD) motif. This protein recruits caspase 2/ICH1 to the cell death signal transduction complex, which includes tumor necrosis factor receptor 1 (TNFR1A) and RIPK1/RIP kinase, and acts in promoting apoptosis. A mutation in this gene was associated with cognitive disability. A related pseudogene is found on chromosome 3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
UniProt Code:	P78560
NCBI GenInfo Identifier:	2498833
NCBI Gene ID:	8738
NCBI Accession:	P78560.1
UniProt Related Accession:	P78560
Molecular Weight:	~ 23kDa
NCBI Full Name:	Death domain-containing protein CRADD
NCBI Synonym Full Names:	CASP2 and RIPK1 domain containing adaptor with death domain
NCBI Official Symbol:	CRADD
NCBI Official Synonym Symbols:	MRT34; RAIDD
NCBI Protein Information:	death domain-containing protein CRADD
UniProt Protein Name:	Death domain-containing protein CRADD
UniProt Synonym Protein Names:	Caspase and RIP adapter with death domain; RIP-associated protein with a death domain
UniProt Gene Name:	CRADD
UniProt Entry Name:	CRADD_HUMAN

Western blot analysis of extracts of various cell lines, using CRADD antibody (CAB1124) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Antibodies

CRADD Antibody (PACO08586)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)