Anti-CPT1A Antibody (CAB20746)

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SKU:
CAB20746
Product Type:
Antibody
Antibody Type:
Secondary Antibody
Host Species:
Goat
Isotype:
Alkaline Phosphatase conjugated IgG

Description

Antibody Name:Anti-CPT1A Antibody (CAB20746)
Antibody SKU:CAB20746
Antibody Size:50µL, 100µL
Application:Western blotting
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 520-720 of human CPT1A (NP_001867.2).
Application:Western blotting
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HepG2, MCF7, Mouse liver, Mouse kidney, Mouse heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 520-720 of human CPT1A (NP_001867.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:WDIP GECQ EVIE TSLN TANL LAND VDFH SFPF VAFG KGII KKCR TSPD AFVQ LALQ LAHY KDMG KFCL TYEA SMTR LFRE GRTE TVRS CTTE SCDF VRAM VDPA QTVE QRLK LFKL ASEK HQHM YRLA MTGS GIDR HLFC LYVV SKYL AVES PFLK EVLS EPWR LSTS QTPQ QQVE LFDL ENNP EYVS SGGG FGPV ADDG Y
Cellular Location:
Calculated MW:86kDa/88kDa
Observed MW:88KDa
Synonyms:CPT1A, CPT1, CPT1-L, L-CPT1
Background:The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Western blot analysis of extracts of various cell lines, using CPT1A antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.Western blot analysis of extracts of various cell lines, using CPT1A antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Western blot analysis of extracts of Mouse heart, using CPT1A antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.Western blot analysis of extracts of Mouse heart, using CPT1A antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
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