Immunology Antibodies 1

Anti-CPSF4 Antibody (CAB10284)

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SKU:
CAB10284
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-CPSF4 Antibody
Antibody SKU:CAB10284
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-244 of human CPSF4 (NP_001075028.1).
Application:WB
Recommended Dilution:WB 1:1000 - 1:2000
Reactivity:Human
Positive Samples:HeLa, Jurkat, 22Rv1
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-244 of human CPSF4 (NP_001075028.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MQEI IASV DHIK FDLE IAVE QQLG AQPL PFPG MDKS GAAV CEFF LKAA CGKG GMCP FRHI SGEK TVVC KHWL RGLC KKGD QCEF LHEY DMTK MPEC YFYS KFGE CSNK ECPF LHID PESK IKDC PWYD RGFC KHGP LCRH RHTR RVIC VNYL VGFC PEGP SCKF MHPR FELP MGTT EQPP LPQQ TQPP AKQR TPQV IGVM QSQN SSAG NRGP RPLE QVTC YKCG EKGH YANR CTKG HLAF LSGQ
Gene ID:10898
Uniprot:O95639
Cellular Location:Nucleus
Calculated MW:27kDa/30kDa
Observed MW:30kDa
Synonyms:CPSF4, CPSF30, NAR, NEB-1, NEB1
Background:Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs. Multiple alternatively spliced transcript variants that encode different isoforms have been described for this gene.
UniProt Protein Function:CPSF4: Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. CPSF4 binds RNA polymers with a preference for poly(U). Belongs to the CPSF4/YTH1 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:RNA processing; Mitochondrial

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: mRNA cleavage and polyadenylation specificity factor complex; nucleoplasm

Molecular Function:protein binding

Biological Process: modification by virus of host mRNA processing; tRNA splicing

NCBI Summary:Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs. Multiple alternatively spliced transcript variants that encode different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:O95639
NCBI GenInfo Identifier:37999487
NCBI Gene ID:10898
NCBI Accession:O95639.1
UniProt Secondary Accession:O95639,Q6FGE6, Q86TF8, Q9BTW6, D6W5S8,
UniProt Related Accession:O95639
Molecular Weight:27,419 Da
NCBI Full Name:Cleavage and polyadenylation specificity factor subunit 4
NCBI Synonym Full Names:cleavage and polyadenylation specific factor 4
NCBI Official Symbol:CPSF4  
NCBI Official Synonym Symbols:NAR; NEB1; NEB-1; CPSF30  
NCBI Protein Information:cleavage and polyadenylation specificity factor subunit 4
UniProt Protein Name:Cleavage and polyadenylation specificity factor subunit 4
UniProt Synonym Protein Names:Cleavage and polyadenylation specificity factor 30 kDa subunit; CPSF 30 kDa subunit; NS1 effector domain-binding protein 1; Neb-1; No arches homolog
Protein Family:Cleavage and polyadenylation specificity factor
UniProt Gene Name:CPSF4  
UniProt Entry Name:CPSF4_HUMAN
Anti-CPSF4 Antibody (CAB10284)Western blot analysis of extracts of various cell lines, using CPSF4 antibody (CAB10284) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
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