Antibody Name:	Anti-CPSF3L Antibody
Antibody SKU:	CAB6566
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 331-600 of human CPSF3L (NP_060341.2).

Application:	WB IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:10 - 1:100 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, SKOV3, MCF7, Mouse spleen, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 331-600 of human CPSF3L (NP_060341.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GQSL QIFR KWAG NEKN MVIM PGYC VQGT VGHK ILSG QRKL EMEG RQVL EVKM QVEY MSFS AHAD AKGI MQLV GQAE PESV LLVH GEAK KMEF LKQK IEQE LRVN CYMP ANGE TVTL PTSP SIPV GISL GLLK REMA QGLL PEAK KPRL LHGT LIMK DSNF RLVS SEQA LKEL GLAE HQLR FTCR VHLH DTRK EQET ALRV YSHL KSVL KDHC VQHL PDGS VTVE SVLL QAAA PSED PGTK VLLV SWTY QDEE LGSF LTSL LKKG LPQA PS
Gene ID:	54973
Uniprot:	Q5TA45
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	56kDa/64kDa/65kDa/67kDa/68kDa
Observed MW:	68kDa

Synonyms:

INTS11, CPSF3L, CPSF73L, INT11, RC-68, RC68

Background:

The Integrator complex contains at least 12 subunits and associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A; MIM 180660) and mediates the 3-prime end processing of small nuclear RNAs U1 (RNU1; MIM 180680) and U2 (RNU2; MIM 180690). INTS11, or CPSF3L, is the catalytic subunit of the Integrator complex (Baillat et al., 2005 [PubMed 16239144]).[supplied by OMIM, Mar 2008]

UniProt Protein Function:	INTS11: Catalytic component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Mediates the snRNAs 3' cleavage. Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. INTS11 subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:RNA processing; Ribonuclease; EC 3.1.27.- Chromosomal Location of Human Ortholog: 1p36.33 Cellular Component: cytoplasm; integrator complex Molecular Function:protein binding; hydrolase activity Biological Process: snRNA processing
NCBI Summary:	The Integrator complex contains at least 12 subunits and associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A; MIM 180660) and mediates the 3-prime end processing of small nuclear RNAs U1 (RNU1; MIM 180680) and U2 (RNU2; MIM 180690). INTS11, or CPSF3L, is the catalytic subunit of the Integrator complex (Baillat et al., 2005 [PubMed 16239144]).[supplied by OMIM, Mar 2008]
UniProt Code:	Q5TA45
NCBI GenInfo Identifier:	118572557
NCBI Gene ID:	54973
NCBI Accession:	Q5TA45.2
UniProt Secondary Accession:	Q5TA45,Q5TA46, Q5TA48, Q5TA52, Q5TA53, Q5TA54, Q7L3D7 Q96HY1, A8K5S2, B3KPR3, B4DM87, G3V1S5,
UniProt Related Accession:	Q5TA45
Molecular Weight:	600
NCBI Full Name:	Integrator complex subunit 11
NCBI Synonym Full Names:	cleavage and polyadenylation specific factor 3-like
NCBI Official Symbol:	CPSF3L
NCBI Official Synonym Symbols:	RC68; INT11; RC-68; INTS11; CPSF73L
NCBI Protein Information:	integrator complex subunit 11; CPSF3-like protein; related to CPSF subunits 68 kDa; protein related to CPSF subunits of 68 kDa; cleavage and polyadenylation-specific factor 3-like protein
UniProt Protein Name:	Integrator complex subunit 11
UniProt Synonym Protein Names:	Cleavage and polyadenylation-specific factor 3-like protein; CPSF3-like protein; Protein related to CPSF subunits of 68 kDa; RC-68
UniProt Gene Name:	CPSF3L
UniProt Entry Name:	INT11_HUMAN

	Western blot analysis of extracts of various cell lines, using CPSF3L antibody (CAB6566) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of HeLa cells using CPSF3L antibody (CAB6566). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug CPSF3L antibody (CAB6566). Western blot was performed from the immunoprecipitate using CPSF3L antibody (CAB6566) at a dilition of 1:500.