Epigenetics & Nuclear Signaling Antibodies 1

Anti-CPSF3 Antibody (CAB12368)

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SKU:
CAB12368
Product Type:
Antibody
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-CPSF3 Antibody
Antibody SKU:CAB12368
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 435-684 of human CPSF3 (NP_057291.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse
Positive Samples:Mouse liver, Mouse spleen
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 435-684 of human CPSF3 (NP_057291.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:YEDN DEVH IEVH NPRN TEAV TLNF RGEK LAKV MGFL ADKK PEQG QRVS GILV KRNF NYHI LSPC DLSN YTDL AMST VKQT QAIP YTGP FNLL CYQL QKLT GDVE ELEI QEKP ALKV FKNI TVIQ EPGM VVLE WLAN PSND MYAD TVTT VILE VQSN PKIR KGAV QKVS KKLE MHVY SKRL EIML QDIF GEDC VSVK DDSI LSVT VDGK TANL NLET RTVE CEEG SEDD ESLR EMVE LAAQ RLYE ALTP VH
Gene ID:51692
Uniprot:Q9UKF6
Cellular Location:Nucleus
Calculated MW:77kDa
Observed MW:73kDa
Synonyms:CPSF3, CPSF-73, CPSF73
Background:This gene encodes a member of the metallo-beta-lactamase family. The encoded protein is a 73kDa subunit of the cleavage and polyadenylation specificity factor and functions as an endonuclease that recognizes the pre-mRNA 3'-cleavage site AAUAAA prior to polyadenylation. It also cleaves after the pre-mRNA sequence ACCCA during histone 3'-end pre-mRNA processing.
UniProt Protein Function:CPSF3: Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP- dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner. Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF3 subfamily.
UniProt Protein Details:

Protein type:EC 3.1.27.-; RNA processing; RNA splicing; RNA-binding; Ribonuclease

Chromosomal Location of Human Ortholog: 2p25.1

Cellular Component: mRNA cleavage and polyadenylation specificity factor complex; nucleoplasm

Molecular Function:5'-3' exonuclease activity; endoribonuclease activity; protein binding; RNA binding

Biological Process: histone mRNA 3'-end processing; mRNA 3'-end processing; mRNA cleavage; mRNA export from nucleus; mRNA polyadenylation; nuclear mRNA splicing, via spliceosome; termination of RNA polymerase II transcription

NCBI Summary:This gene encodes a member of the metallo-beta-lactamase family. The encoded protein is a 73kDa subunit of the cleavage and polyadenylation specificity factor and functions as an endonuclease that recognizes the pre-mRNA 3'-cleavage site AAUAAA prior to polyadenylation. It also cleaves after the pre-mRNA sequence ACCCA during histone 3'-end pre-mRNA processing. [provided by RefSeq, Oct 2012]
UniProt Code:Q9UKF6
NCBI GenInfo Identifier:18203503
NCBI Gene ID:51692
NCBI Accession:Q9UKF6.1
UniProt Secondary Accession:Q9UKF6,O14769, Q53RS2, Q96F36,
UniProt Related Accession:Q9UKF6
Molecular Weight:77kDa
NCBI Full Name:Cleavage and polyadenylation specificity factor subunit 3
NCBI Synonym Full Names:cleavage and polyadenylation specific factor 3
NCBI Official Symbol:CPSF3  
NCBI Official Synonym Symbols:CPSF73; CPSF-73  
NCBI Protein Information:cleavage and polyadenylation specificity factor subunit 3
UniProt Protein Name:Cleavage and polyadenylation specificity factor subunit 3
UniProt Synonym Protein Names:Cleavage and polyadenylation specificity factor 73 kDa subunit; CPSF 73 kDa subunit; mRNA 3'-end-processing endonuclease CPSF-73
Protein Family:Cleavage and polyadenylation specificity factor
UniProt Gene Name:CPSF3  
Anti-CPSF3 Antibody (CAB12368)Western blot analysis of extracts of various cell lines, using CPSF3 antibody (CAB12368) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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