Name: Anti-CPN1 Antibody (CAB7887)
Brand: Cell Biology Antibodies 11
SKU: CAB7887
Availability: OutOfStock

Antibody Name:	Anti-CPN1 Antibody
Antibody SKU:	CAB7887
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 209-458 of human CPN1 (NP_001299.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, Raji, Mouse liver, Mouse pancreas, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 209-458 of human CPN1 (NP_001299.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FVLS ANLH GGAV VANY PYDK SFEH RVRG VRRT ASTP TPDD KLFQ KLAK VYSY AHGW MFQG WNCG DYFP DGIT NGAS WYSL SKGM QDFN YLHT NCFE ITLE LSCD KFPP EEEL QREW LGNR EALI QFLE QVHQ GIKG MVLD ENYN NLAN AVIS VSGI NHDV TSGD HGDY FRLL LPGI YTVS ATAP GYDP ETVT VTVG PAEP TLVN FHLK RSIP QVSP VRRA PSRR HGVR AKVQ PQAR KKEM EMRQ LQRG PA
Gene ID:	1369
Uniprot:	P15169
Cellular Location:	Secreted, extracellular space
Calculated MW:	52kDa
Observed MW:	52kDa

Synonyms:

CPN1, CPN, SCPN

Background:

Carboxypeptidase N is a plasma metallo-protease that cleaves basic amino acids from the C terminal of peptides and proteins. The enzyme is important in the regulation of peptides like kinins and anaphylatoxins, and has also been known as kininase-1 and anaphylatoxin inactivator. This enzyme is a tetramer comprised of two identical regulatory subunits and two identical catalytic subunits; this gene encodes the catalytic subunit. Mutations in this gene can be associated with angioedema or chronic urticaria resulting from carboxypeptidase N deficiency.

UniProt Protein Function:	CPN1: Protects the body from potent vasoactive and inflammatory peptides containing C-terminal Arg or Lys (such as kinins or anaphylatoxins) which are released into the circulation. Defects in CPN1 are the cause of carboxypeptidase N deficiency (CPND). Patients affected present some combination of angioedema or chronic urticaria, as well as hay fever or astma, and have also slightly depressed serum carboxy peptidase N, suggestive of autosomal recessive inheritance of this disorder. Belongs to the peptidase M14 family.
UniProt Protein Details:	Protein type:Secreted; EC 3.4.17.3; Protease; Secreted, signal peptide Chromosomal Location of Human Ortholog: 10q24.2 Molecular Function:serine carboxypeptidase activity Biological Process: peptide metabolic process; protein processing Disease: Carboxypeptidase N Deficiency
NCBI Summary:	Carboxypeptidase N is a plasma metallo-protease that cleaves basic amino acids from the C terminal of peptides and proteins. The enzyme is important in the regulation of peptides like kinins and anaphylatoxins, and has also been known as kininase-1 and anaphylatoxin inactivator. This enzyme is a tetramer comprised of two identical regulatory subunits and two identical catalytic subunits; this gene encodes the catalytic subunit. Mutations in this gene can be associated with angioedema or chronic urticaria resulting from carboxypeptidase N deficiency. [provided by RefSeq, Jul 2008]
UniProt Code:	P15169
NCBI GenInfo Identifier:	115896
NCBI Gene ID:	1369
NCBI Accession:	P15169.1
UniProt Secondary Accession:	P15169,B1AP59,
UniProt Related Accession:	P15169
Molecular Weight:	52,286 Da
NCBI Full Name:	Carboxypeptidase N catalytic chain
NCBI Synonym Full Names:	carboxypeptidase N subunit 1
NCBI Official Symbol:	CPN1
NCBI Official Synonym Symbols:	CPN; SCPN
NCBI Protein Information:	carboxypeptidase N catalytic chain
UniProt Protein Name:	Carboxypeptidase N catalytic chain
UniProt Synonym Protein Names:	Anaphylatoxin inactivator; Arginine carboxypeptidase; Carboxypeptidase N polypeptide 1; Carboxypeptidase N small subunit; Kininase-1; Lysine carboxypeptidase; Plasma carboxypeptidase B; Serum carboxypeptidase N; SCPN
Protein Family:	Carboxypeptidase
UniProt Gene Name:	CPN1
UniProt Entry Name:	CBPN_HUMAN

Western blot analysis of extracts of various cell lines, using CPN1 antibody (CAB7887) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s.

Antibodies

CPN1 Antibody (PACO06373)

CPN1 Antibody (PACO08570)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)