Cell Biology Antibodies 8

Anti-COX5B Antibody (CAB2640)

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SKU:
CAB2640
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-COX5B Antibody
Antibody SKU:CAB2640
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 32-129 of human COX5B (NP_001853.2).
Application:WB IHC IF IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 IP 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:HepG2, 22Rv1, SW480, HeLa, A-549, Mouse heart, Mouse kidney, Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 32-129 of human COX5B (NP_001853.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ASGG GVPT DEEQ ATGL EREI MLAA KKGL DPYN VLAP KGAS GTRE DPNL VPSI SNKR IVGC ICEE DNTS VVWF WLHK GEAQ RCPR CGAH YKLV PQQL AH
Gene ID:1329
Uniprot:P10606
Cellular Location:Mitochondrion inner membrane
Calculated MW:13kDa
Observed MW:14kDa
Synonyms:COX5B, COXVB
Background:Cytochrome C oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Vb of the human mitochondrial respiratory chain enzyme.
UniProt Protein Function:COX5B: This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. Belongs to the cytochrome c oxidase subunit 5B family.
UniProt Protein Details:

Protein type:Energy Metabolism - oxidative phosphorylation; EC 1.9.3.1; Oxidoreductase; Mitochondrial

Chromosomal Location of Human Ortholog: 2q11.2

Cellular Component: mitochondrion; mitochondrial inner membrane

Molecular Function:cytochrome-c oxidase activity; metal ion binding

Biological Process: cellular metabolic process; respiratory gaseous exchange; transmembrane transport

NCBI Summary:Cytochrome C oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Vb of the human mitochondrial respiratory chain enzyme. [provided by RefSeq, Jul 2008]
UniProt Code:P10606
NCBI GenInfo Identifier:20141332
NCBI Gene ID:1329
NCBI Accession:P10606.2
UniProt Related Accession:P10606
Molecular Weight:14kDa
NCBI Full Name:Cytochrome c oxidase subunit 5B, mitochondrial
NCBI Synonym Full Names:cytochrome c oxidase subunit 5B
NCBI Official Symbol:COX5B  
NCBI Official Synonym Symbols:COXVB  
NCBI Protein Information:cytochrome c oxidase subunit 5B, mitochondrial
UniProt Protein Name:Cytochrome c oxidase subunit 5B, mitochondrial
UniProt Synonym Protein Names:Cytochrome c oxidase polypeptide Vb
Protein Family:Cytochrome c oxidase polypeptide
UniProt Gene Name:COX5B  
UniProt Entry Name:COX5B_HUMAN
Anti-COX5B Antibody (CAB2640)Western blot analysis of extracts of various cell lines, using COX5B antibody (CAB2640) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
Anti-COX5B Antibody (CAB2640)Immunohistochemistry of paraffin-embedded human colon using COX5B Rabbit pAb (CAB2640) at dilution of 1:100 (40x lens).
Anti-COX5B Antibody (CAB2640)Immunohistochemistry of paraffin-embedded mouse kidney using COX5B Rabbit pAb (CAB2640) at dilution of 1:100 (40x lens).
Anti-COX5B Antibody (CAB2640)Immunohistochemistry of paraffin-embedded rat kidney using COX5B Rabbit pAb (CAB2640) at dilution of 1:100 (40x lens).
Anti-COX5B Antibody (CAB2640)Immunofluorescence analysis of C6 cells using COX5B antibody (CAB2640) at dilution of 1:100. Blue: DAPI for nuclear staining.
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