Antibody Name:	Anti-COX5B Antibody
Antibody SKU:	CAB2640
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 32-129 of human COX5B (NP_001853.2).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 IP 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, 22Rv1, SW480, HeLa, A-549, Mouse heart, Mouse kidney, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 32-129 of human COX5B (NP_001853.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ASGG GVPT DEEQ ATGL EREI MLAA KKGL DPYN VLAP KGAS GTRE DPNL VPSI SNKR IVGC ICEE DNTS VVWF WLHK GEAQ RCPR CGAH YKLV PQQL AH
Gene ID:	1329
Uniprot:	P10606
Cellular Location:	Mitochondrion inner membrane
Calculated MW:	13kDa
Observed MW:	14kDa

Synonyms:

COX5B, COXVB

Background:

Cytochrome C oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Vb of the human mitochondrial respiratory chain enzyme.

UniProt Protein Function:	COX5B: This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. Belongs to the cytochrome c oxidase subunit 5B family.
UniProt Protein Details:	Protein type:Energy Metabolism - oxidative phosphorylation; EC 1.9.3.1; Oxidoreductase; Mitochondrial Chromosomal Location of Human Ortholog: 2q11.2 Cellular Component: mitochondrion; mitochondrial inner membrane Molecular Function:cytochrome-c oxidase activity; metal ion binding Biological Process: cellular metabolic process; respiratory gaseous exchange; transmembrane transport
NCBI Summary:	Cytochrome C oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Vb of the human mitochondrial respiratory chain enzyme. [provided by RefSeq, Jul 2008]
UniProt Code:	P10606
NCBI GenInfo Identifier:	20141332
NCBI Gene ID:	1329
NCBI Accession:	P10606.2
UniProt Related Accession:	P10606
Molecular Weight:	14kDa
NCBI Full Name:	Cytochrome c oxidase subunit 5B, mitochondrial
NCBI Synonym Full Names:	cytochrome c oxidase subunit 5B
NCBI Official Symbol:	COX5B
NCBI Official Synonym Symbols:	COXVB
NCBI Protein Information:	cytochrome c oxidase subunit 5B, mitochondrial
UniProt Protein Name:	Cytochrome c oxidase subunit 5B, mitochondrial
UniProt Synonym Protein Names:	Cytochrome c oxidase polypeptide Vb
Protein Family:	Cytochrome c oxidase polypeptide
UniProt Gene Name:	COX5B
UniProt Entry Name:	COX5B_HUMAN

	Western blot analysis of extracts of various cell lines, using COX5B antibody (CAB2640) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human colon using COX5B Rabbit pAb (CAB2640) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using COX5B Rabbit pAb (CAB2640) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat kidney using COX5B Rabbit pAb (CAB2640) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using COX5B antibody (CAB2640) at dilution of 1:100. Blue: DAPI for nuclear staining.