Cell Biology Antibodies 6

Anti-COX11 Antibody (CAB16290)

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SKU:
CAB16290
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-COX11 Antibody
Antibody SKU:CAB16290
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 140-240 of human COX11 (NP_004366.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 140-240 of human COX11 (NP_004366.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ENMV PVKD RIIK ISFN ADVH ASLQ WNFR PQQT EIYV VPGE TALA FYRA KNPT DKPV IGIS TYNI VPFE AGQY FNKI QCFC FEEQ RLNP QEEV DMPV FFYI D
Gene ID:1353
Uniprot:Q9Y6N1
Cellular Location:Intermembrane side, Mitochondrion inner membrane, Single-pass membrane protein
Calculated MW:26kDa/31kDa
Observed MW:
Synonyms:COX11, COX11P
Background:Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes a protein which is not a structural subunit, but may be a heme A biosynthetic enzyme involved in COX formation, according to the yeast mutant studies. However, the studies in Rhodobacter sphaeroides suggest that this gene is not required for heme A biosynthesis, but required for stable formation of the Cu(B) and magnesium centers of COX. This human protein is predicted to contain a transmembrane domain localized in the mitochondrial inner membrane. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene has been found on chromosome 6.
UniProt Protein Function:COX11: Exerts its effect at some terminal stage of cytochrome c oxidase synthesis, probably by being involved in the insertion of the copper B into subunit I. Belongs to the COX11/CtaG family.
UniProt Protein Details:

Protein type:Mitochondrial; Oxidoreductase; Energy Metabolism - oxidative phosphorylation; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17q22

Cellular Component: mitochondrion

Molecular Function:cytochrome-c oxidase activity; electron carrier activity

Biological Process: respiratory chain complex IV assembly; respiratory gaseous exchange

NCBI Summary:Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes a protein which is not a structural subunit, but may be a heme A biosynthetic enzyme involved in COX formation, according to the yeast mutant studies. However, the studies in Rhodobacter sphaeroides suggest that this gene is not required for heme A biosynthesis, but required for stable formation of the Cu(B) and magnesium centers of COX. This human protein is predicted to contain a transmembrane domain localized in the mitochondrial inner membrane. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene has been found on chromosome 6. [provided by RefSeq, Jun 2009]
UniProt Code:Q9Y6N1
NCBI GenInfo Identifier:60416378
NCBI Gene ID:1353
NCBI Accession:Q9Y6N1.3
UniProt Secondary Accession:Q9Y6N1,Q6FHB7, Q9BRX0, Q9UME8, D3DTY5, I3L220,
UniProt Related Accession:Q9Y6N1
Molecular Weight:26,133 Da
NCBI Full Name:Cytochrome c oxidase assembly protein COX11, mitochondrial
NCBI Synonym Full Names:COX11, cytochrome c oxidase copper chaperone
NCBI Official Symbol:COX11  
NCBI Official Synonym Symbols:COX11P  
NCBI Protein Information:cytochrome c oxidase assembly protein COX11, mitochondrial
UniProt Protein Name:Cytochrome c oxidase assembly protein COX11, mitochondrial
Protein Family:Cytochrome c oxidase assembly protein
UniProt Gene Name:COX11  
UniProt Entry Name:COX11_HUMAN
Anti-COX11 Antibody (CAB16290)Western blot analysis of extracts of various cell lines, using COX11 antibody (CAB16290) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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