Name: Anti-COX11 Antibody (CAB16290)
Brand: Cell Biology Antibodies 6
SKU: CAB16290
Availability: OutOfStock

Antibody Name:	Anti-COX11 Antibody
Antibody SKU:	CAB16290
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 140-240 of human COX11 (NP_004366.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Mouse, Rat
Positive Samples:

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 140-240 of human COX11 (NP_004366.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ENMV PVKD RIIK ISFN ADVH ASLQ WNFR PQQT EIYV VPGE TALA FYRA KNPT DKPV IGIS TYNI VPFE AGQY FNKI QCFC FEEQ RLNP QEEV DMPV FFYI D
Gene ID:	1353
Uniprot:	Q9Y6N1
Cellular Location:	Intermembrane side, Mitochondrion inner membrane, Single-pass membrane protein
Calculated MW:	26kDa/31kDa
Observed MW:

Synonyms:

COX11, COX11P

Background:

Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes a protein which is not a structural subunit, but may be a heme A biosynthetic enzyme involved in COX formation, according to the yeast mutant studies. However, the studies in Rhodobacter sphaeroides suggest that this gene is not required for heme A biosynthesis, but required for stable formation of the Cu(B) and magnesium centers of COX. This human protein is predicted to contain a transmembrane domain localized in the mitochondrial inner membrane. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene has been found on chromosome 6.

UniProt Protein Function:	COX11: Exerts its effect at some terminal stage of cytochrome c oxidase synthesis, probably by being involved in the insertion of the copper B into subunit I. Belongs to the COX11/CtaG family.
UniProt Protein Details:	Protein type:Mitochondrial; Oxidoreductase; Energy Metabolism - oxidative phosphorylation; Membrane protein, integral Chromosomal Location of Human Ortholog: 17q22 Cellular Component: mitochondrion Molecular Function:cytochrome-c oxidase activity; electron carrier activity Biological Process: respiratory chain complex IV assembly; respiratory gaseous exchange
NCBI Summary:	Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes a protein which is not a structural subunit, but may be a heme A biosynthetic enzyme involved in COX formation, according to the yeast mutant studies. However, the studies in Rhodobacter sphaeroides suggest that this gene is not required for heme A biosynthesis, but required for stable formation of the Cu(B) and magnesium centers of COX. This human protein is predicted to contain a transmembrane domain localized in the mitochondrial inner membrane. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene has been found on chromosome 6. [provided by RefSeq, Jun 2009]
UniProt Code:	Q9Y6N1
NCBI GenInfo Identifier:	60416378
NCBI Gene ID:	1353
NCBI Accession:	Q9Y6N1.3
UniProt Secondary Accession:	Q9Y6N1,Q6FHB7, Q9BRX0, Q9UME8, D3DTY5, I3L220,
UniProt Related Accession:	Q9Y6N1
Molecular Weight:	26,133 Da
NCBI Full Name:	Cytochrome c oxidase assembly protein COX11, mitochondrial
NCBI Synonym Full Names:	COX11, cytochrome c oxidase copper chaperone
NCBI Official Symbol:	COX11
NCBI Official Synonym Symbols:	COX11P
NCBI Protein Information:	cytochrome c oxidase assembly protein COX11, mitochondrial
UniProt Protein Name:	Cytochrome c oxidase assembly protein COX11, mitochondrial
Protein Family:	Cytochrome c oxidase assembly protein
UniProt Gene Name:	COX11
UniProt Entry Name:	COX11_HUMAN

Western blot analysis of extracts of various cell lines, using COX11 antibody (CAB16290) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Antibodies

COX11 Antibody (PACO03472)

COX11 Antibody (PACO08541)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)