Cell Biology Antibodies 3

Anti-COX IV Antibody (CAB1263)

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SKU:
CAB1263
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-COX IV Antibody
Antibody SKU:CAB1263
Antibody Size:100µL
Application:WB IHC IF IP FC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human COX IV
Application:WB IHC IF IP FC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:20 - 1:50 IP 1:20 - 1:50 FC 1:20 - 1:50
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7
Immunogen:Recombinant protein of human COX IV
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:1327
Uniprot:P13073
Cellular Location:Mitochondrion inner membrane
Calculated MW:19kDa
Observed MW:20kDa
Synonyms:COX4I1, COX IV-1, COX4, COX4-1, COXIV, COXIV-1, COX IV
Background:Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. Pseudogenes related to this gene are located on chromosomes 13 and 14. Alternative splicing results in multiple transcript variants encoding different isoforms.
UniProt Protein Function:COX4I1: This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. Ubiquitous. Belongs to the cytochrome c oxidase IV family.
UniProt Protein Details:

Protein type:Membrane protein, integral; Energy Metabolism - oxidative phosphorylation; Mitochondrial; EC 1.9.3.1; Oxidoreductase

Chromosomal Location of Human Ortholog: 16q24.1

Cellular Component: membrane; mitochondrial inner membrane; mitochondrial respiratory chain complex IV; mitochondrion; nucleus

Molecular Function:protein binding

Biological Process: generation of precursor metabolites and energy; mitochondrial electron transport, cytochrome c to oxygen

NCBI Summary:Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. Pseudogenes related to this gene are located on chromosomes 13 and 14. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]
UniProt Code:P13073
NCBI GenInfo Identifier:117086
NCBI Gene ID:1327
NCBI Accession:P13073.1
UniProt Secondary Accession:P13073,Q6P666, B2R4J2, D3DUM7,
UniProt Related Accession:P13073
Molecular Weight:19,577 Da
NCBI Full Name:Cytochrome c oxidase subunit 4 isoform 1, mitochondrial
NCBI Synonym Full Names:cytochrome c oxidase subunit 4I1
NCBI Official Symbol:COX4I1  
NCBI Official Synonym Symbols:COX4; COXIV; COX4-1; COXIV-1; COX IV-1  
NCBI Protein Information:cytochrome c oxidase subunit 4 isoform 1, mitochondrial
UniProt Protein Name:Cytochrome c oxidase subunit 4 isoform 1, mitochondrial
UniProt Synonym Protein Names:Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit IV isoform 1; COX IV-1
Protein Family:Cytochrome c oxidase
UniProt Gene Name:COX4I1  
UniProt Entry Name:COX41_HUMAN
Anti-COX IV Antibody (CAB1263)Western blot analysis of extracts of MCF-7 cells, using COX IV antibody (CAB1263). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
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