Antibody Name:	Anti-Coronin-1A Antibody
Antibody SKU:	CAB9300
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 362-461 of human CORO1A (NP_001180262.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HL-60, SW480, Mouse brain, Mouse lung, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 362-461 of human CORO1A (NP_001180262.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DLYP PTAG PDPA LTAE EWLG GRDA GPLL ISLK DGYV PPKS RELR VNRG LDTG RRRA APEA SGTP SSDA VSRL EEEM RKLQ ATVQ ELQK RLDR LEET VQAK
Gene ID:	11151
Uniprot:	P31146
Cellular Location:	Cytoplasm, Cytoplasmic vesicle, cell cortex, cytoskeleton, phagosome membrane
Calculated MW:	51kDa
Observed MW:	51kDa

Synonyms:

CORO1A, CLABP, CLIPINA, HCORO1, IMD8, TACO, p57

Background:

This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Alternative splicing results in multiple transcript variants. A related pseudogene has been defined on chromosome 16.

UniProt Protein Function:	coronin 1A: May be a crucial component of the cytoskeleton of highly motile cells, functioning both in the invagination of large pieces of plasma membrane, as well as in forming protrusions of the plasma membrane involved in cell locomotion. In mycobacteria- infected cells, its retention on the phagosomal membrane prevents fusion between phagosomes and lysosomes. Belongs to the WD repeat coronin family.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Cytoskeletal Chromosomal Location of Human Ortholog: 16p11.2 Cellular Component: actin filament; axon; cortical actin cytoskeleton; cytoplasm; early endosome; immunological synapse; intercellular junction; lamellipodium; membrane; nucleus; phagocytic cup; phagocytic vesicle; phagocytic vesicle membrane; plasma membrane; protein complex Molecular Function:actin binding; actin filament binding; actin monomer binding; cytoskeletal protein binding; myosin heavy chain binding; phosphoinositide 3-kinase binding; protein binding; protein C-terminus binding; protein homodimerization activity Biological Process: actin cytoskeleton organization and biogenesis; actin filament organization; calcium ion transport; cell motility; cell-substrate adhesion; formation of immunological synapse; homeostasis of number of cells within a tissue; innate immune response; leukocyte chemotaxis; natural killer cell degranulation; negative regulation of actin nucleation; negative regulation of neuron apoptosis; negative regulation of vesicle fusion; phagocytosis; phagolysosome formation; positive chemotaxis; positive regulation of cell migration; positive regulation of T cell proliferation; regulation of actin cytoskeleton organization and biogenesis; regulation of cell shape; regulation of release of sequestered calcium ion into cytosol; T cell homeostasis; uropod organization and biogenesis Disease: Immunodeficiency 8
NCBI Summary:	This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Alternative splicing results in multiple transcript variants. A related pseudogene has been defined on chromosome 16. [provided by RefSeq, Sep 2010]
UniProt Code:	P31146
NCBI GenInfo Identifier:	1706004
NCBI Gene ID:	11151
NCBI Accession:	P31146.4
UniProt Secondary Accession:	P31146,Q2YD73, B2RBL1,
UniProt Related Accession:	P31146
Molecular Weight:	51,026 Da
NCBI Full Name:	Coronin-1A
NCBI Synonym Full Names:	coronin 1A
NCBI Official Symbol:	CORO1A
NCBI Official Synonym Symbols:	p57; IMD8; TACO; CLABP; HCORO1; CLIPINA
NCBI Protein Information:	coronin-1A
UniProt Protein Name:	Coronin-1A
UniProt Synonym Protein Names:	Coronin-like protein A; Clipin-A; Coronin-like protein p57; Tryptophan aspartate-containing coat protein; TACO
Protein Family:	Coronin
UniProt Gene Name:	CORO1A
UniProt Entry Name:	COR1A_HUMAN

	Western blot analysis of extracts of various cell lines, using CORO1A antibody (CAB9300) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded rat spleen using CORO1A Rabbit pAb (CAB9300) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human thyroid cancer using CORO1A Rabbit pAb (CAB9300) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophageal cancer using CORO1A Rabbit pAb (CAB9300) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse liver using CORO1A Rabbit pAb (CAB9300) at dilution of 1:100 (40x lens).