Description
| Product Name: | CoREST/RCOR1 Rabbit mAb |
| Product Code: | CAB3568 |
| Size: | 20uL, 50uL, 100uL |
| Synonyms: | COREST, RCOR |
| Applications: | WB, IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthesized peptide derived from human CoREST/RCOR1 |
| Applications: | WB, IHC |
| Recommended Dilutions: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HT-29, HepG2, C2C12 |
| Immunogen: | A synthesized peptide derived from human CoREST/RCOR1 |
| Purification Method: | Affinity purification |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 23186 |
| Uniprot: | Q9UKL0 |
| Cellular Location: | Nucleus |
| Calculated MW: | 66kDa |
| Observed MW: | 65kDa |
| UniProt Protein Function: | RCOR1: Essential component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. In the BHC complex, it serves as a molecular beacon for the recruitment of molecular machinery, including MeCP2 and SUV39H1, that imposes silencing across a chromosomal interval. Plays a central role in demethylation of Lys-4 of histone H3 by promoting demethylase activity of KDM1A on core histones and nucleosomal substrates. It also protects KDM1A from the proteasome. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development and controls hematopoietic differentiation. Belongs to the CoREST family. |
| UniProt Protein Details: | Protein type:Transcription, coactivator/corepressor; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 14q32.31 Cellular Component: nucleoplasm; nucleus; transcription factor complex; transcriptional repressor complex Molecular Function:histone deacetylase activity; protein binding; transcription corepressor activity; transcription factor binding Biological Process: blood coagulation; negative regulation of transcription, DNA-dependent |
| NCBI Summary: | This gene encodes a protein that is well-conserved, downregulated at birth, and with a specific role in determining neural cell differentiation. The encoded protein binds to the C-terminal domain of REST (repressor element-1 silencing transcription factor). [provided by RefSeq, Aug 2011] |
| UniProt Code: | Q9UKL0 |
| NCBI GenInfo Identifier: | 74762776 |
| NCBI Gene ID: | 23186 |
| NCBI Accession: | Q9UKL0.1 |
| UniProt Secondary Accession: | Q9UKL0,Q15044, Q6P2I9, Q86VG5, |
| UniProt Related Accession: | Q9UKL0 |
| Molecular Weight: | 53,028 Da |
| NCBI Full Name: | REST corepressor 1 |
| NCBI Synonym Full Names: | REST corepressor 1 |
| NCBI Official Symbol: | RCOR1 |
| NCBI Official Synonym Symbols: | RCOR; COREST |
| NCBI Protein Information: | REST corepressor 1 |
| UniProt Protein Name: | REST corepressor 1 |
| UniProt Synonym Protein Names: | Protein CoREST |
| Protein Family: | REST corepressor |
| UniProt Gene Name: | RCOR1 |
| UniProt Entry Name: | RCOR1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CoREST/RCOR1 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min. |
 | Western blot analysis of extracts of C2C12 cells, using CoREST/RCOR1 Rabbit mAb at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min. |
 | Immunohistochemistry of paraffin-embedded rat lung using CoREST/RCOR1 Rabbit mAb at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with IHC staining protocol. |
 | Immunohistochemistry of paraffin-embedded human appendix using CoREST/RCOR1 Rabbit mAb at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with IHC staining protocol. |
 | Immunohistochemistry of paraffin-embedded mouse brain using CoREST/RCOR1 Rabbit mAb at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with IHC staining protocol. |
 | Immunoprecipitation analysis of 300ug extracts of THP-1 cells using 3ug CoREST/RCOR1 antibody at a dilition of 1:1000. |
 | Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using CoREST/RCOR1 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input. |
