Cell Biology Antibodies 2

Anti-COPZ1 Antibody (CAB12148)

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SKU:
CAB12148
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-COPZ1 Antibody
Antibody SKU:CAB12148
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-177 of human COPZ1 (NP_057141.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:HT-29, LO2, HeLa, Mouse kidney, Mouse testis, Mouse spleen
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-177 of human COPZ1 (NP_057141.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MEAL ILEP SLYT VKAI LILD NDGD RLFA KYYD DTYP SVKE QKAF EKNI FNKT HRTD SEIA LLEG LTVV YKSS IDLY FYVI GSSY ENEL MLMA VLNC LFDS LSQM LRKN VEKR ALLE NMEG LFLA VDEI VDGG VILE SDPQ QVVH RVAL RGED VPLT EQTV SQVL QSAK EQIK WSLL R
Gene ID:22818
Uniprot:P61923
Cellular Location:COPI-coated vesicle membrane, Cytoplasm, Cytoplasmic side, Cytoplasmic vesicle, Golgi apparatus membrane, Peripheral membrane protein
Calculated MW:14kDa/17kDa/18kDa/20kDa/21kDa
Observed MW:19kDa
Synonyms:COPZ1, CGI-120, COPZ, HSPC181, zeta-COP, zeta1-COP
Background:This gene encodes a subunit of the cytoplasmic coatamer protein complex, which is involved in autophagy and intracellular protein trafficking. The coatomer protein complex is comprised of seven subunits and functions as the coat protein of coat protein complex (COP)I-vesicles. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:COPZ1: The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Belongs to the adaptor complexes small subunit family.
UniProt Protein Details:

Protein type:Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: COPI vesicle coat; cytosol; endoplasmic reticulum membrane; Golgi membrane; transport vesicle

Biological Process: ER to Golgi vesicle-mediated transport; intra-Golgi vesicle-mediated transport; retrograde vesicle-mediated transport, Golgi to ER

NCBI Summary:This gene encodes a subunit of the cytoplasmic coatamer protein complex, which is involved in autophagy and intracellular protein trafficking. The coatomer protein complex is comprised of seven subunits and functions as the coat protein of coat protein complex (COP)I-vesicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]
UniProt Code:P61923
NCBI GenInfo Identifier:48428830
NCBI Gene ID:22818
NCBI Accession:P61923.1
UniProt Secondary Accession:P61923,Q549N6, Q9Y3C3, B4DDX8, B4DHZ0, F8VS17, F8VWL5
UniProt Related Accession:P61923
Molecular Weight:20kDa
NCBI Full Name:Coatomer subunit zeta-1
NCBI Synonym Full Names:coatomer protein complex subunit zeta 1
NCBI Official Symbol:COPZ1  
NCBI Official Synonym Symbols:COPZ; CGI-120; HSPC181; zeta-COP; zeta1-COP  
NCBI Protein Information:coatomer subunit zeta-1
UniProt Protein Name:Coatomer subunit zeta-1
UniProt Synonym Protein Names:Zeta-1-coat protein; Zeta-1 COP
Protein Family:Coatomer
UniProt Gene Name:COPZ1  
Anti-COPZ1 Antibody (CAB12148)Western blot analysis of extracts of various cell lines, using COPZ1 antibody (CAB12148) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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