Antibody Name:	Anti-COPS8 Antibody
Antibody SKU:	CAB12745
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-209 of human COPS8 (NP_006701.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, A375, HeLa, HepG2, Mouse brain, Mouse heart, Mouse liver, Mouse testis, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-209 of human COPS8 (NP_006701.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MPVA VMAE SAFS FKKL LDQC ENQE LEAP GGIA TPPV YGQL LALY LLHN DMNN ARYL WKRI PPAI KSAN SELG GIWS VGQR IWQR DFPG IYTT INAH QWSE TVQP IMEA LRDA TRRR AFAL VSQA YTSI IADD FAAF VGLP VEEA VKGI LEQG WQAD STTR MVLP RKPV AGAL DVSF NKFI PLSE PAPV PPIP NEQQ LARL TDYV AFLE N
Gene ID:	10920
Uniprot:	Q99627
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	17kDa/23kDa
Observed MW:	23kDa

Synonyms:

COPS8, COP9, CSN8, SGN8

Background:

The protein encoded by this gene is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. The structure and function of COP9 signalosome is similar to that of the 19S regulatory particle of 26S proteasome. COP9 signalosome has been shown to interact with SCF-type E3 ubiquitin ligases and act as a positive regulator of E3 ubiquitin ligases. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

UniProt Protein Function:	COPS8: Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Belongs to the CSN8 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 2q37.3 Cellular Component: cytosol; nucleoplasm; nucleus; perinuclear region of cytoplasm; signalosome Molecular Function:protein binding Biological Process: activation of NF-kappaB-inducing kinase; negative regulation of cell proliferation; nucleotide-excision repair, DNA damage recognition; protein deneddylation; transcription-coupled nucleotide-excision repair
NCBI Summary:	The protein encoded by this gene is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. The structure and function of COP9 signalosome is similar to that of the 19S regulatory particle of 26S proteasome. COP9 signalosome has been shown to interact with SCF-type E3 ubiquitin ligases and act as a positive regulator of E3 ubiquitin ligases. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
UniProt Code:	Q99627
NCBI GenInfo Identifier:	55976572
NCBI Gene ID:	10920
NCBI Accession:	Q99627.1
UniProt Secondary Accession:	Q99627,Q53QS9, A8K1H6,
UniProt Related Accession:	Q99627
Molecular Weight:	23kDa
NCBI Full Name:	COP9 signalosome complex subunit 8
NCBI Synonym Full Names:	COP9 signalosome subunit 8
NCBI Official Symbol:	COPS8
NCBI Official Synonym Symbols:	COP9; CSN8; SGN8
NCBI Protein Information:	COP9 signalosome complex subunit 8
UniProt Protein Name:	COP9 signalosome complex subunit 8
UniProt Synonym Protein Names:	COP9 homolog; hCOP9; JAB1-containing signalosome subunit 8
UniProt Gene Name:	COPS8

	Western blot analysis of extracts of various cell lines, using COPS8 antibody (CAB12745) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunofluorescence analysis of U2OS cells using COPS8 Rabbit pAb (CAB12745) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using COPS8 Rabbit pAb (CAB12745) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of H9C2 cells using COPS8 Rabbit pAb (CAB12745) at dilution of 1:100. Blue: DAPI for nuclear staining.