Antibody Name:	Anti-COPS6 Antibody
Antibody SKU:	CAB7072
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 58-327 of human COPS6 (NP_006824.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, Jurkat, Mouse heart, Mouse brain, Mouse liver, Rat liver, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 58-327 of human COPS6 (NP_006824.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MRSQ EGRP VQVI GALI GKQE GRNI EVMN SFEL LSHT VEEK IIID KEYY YTKE EQFK QVFK ELEF LGWY TTGG PPDP SDIH VHKQ VCEI IESP LFLK LNPM TKHT DLPV SVFE SVID IING EATM LFAE LTYT LATE EAER IGVD HVAR MTAT GSGE NSTV AEHL IAQH SAIK MLHS RVKL ILEY VKAS EAGE VPFN HEIL REAY ALCH CLPV LSTD KFKT DFYD QCND VGLM AYLG TITK TCNT MNQF VNKF NVLY DRQG IGRR MRGL FF
Gene ID:	10980
Uniprot:	Q7L5N1
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	36kDa
Observed MW:	36kDa

Synonyms:

COPS6, CSN6, MOV34-34KD

Background:

The protein encoded by this gene is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. The structure and function of COP9 signalosome is similar to that of the 19S regulatory particle of 26S proteasome. COP9 signalosome has been shown to interact with SCF-type E3 ubiquitin ligases and act as a positive regulator of E3 ubiquitin ligases. This protein belongs to translation initiation factor 3 (eIF3) superfamily. It is involved in the regulation of cell cycle and likely to be a cellular cofactor for HIV-1 accessory gene product Vpr.

UniProt Protein Function:	COPS6: Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Has some glucocorticoid receptor- responsive activity. Stabilizes RFWD2/COP1 through reducing RFWD2 auto-ubiquitination and decelerating RFWD2 turnover rate, hence regulates the ubiquitination of RFWD2 targets. Belongs to the peptidase M67A family. CSN6 subfamily.
UniProt Protein Details:	Protein type:Cell cycle regulation Chromosomal Location of Human Ortholog: 7q22.1 Cellular Component: nucleoplasm; signalosome; cytoplasm Molecular Function:protein binding Biological Process: cullin deneddylation; viral reproduction
NCBI Summary:	The protein encoded by this gene is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. The structure and function of COP9 signalosome is similar to that of the 19S regulatory particle of 26S proteasome. COP9 signalosome has been shown to interact with SCF-type E3 ubiquitin ligases and act as a positive regulator of E3 ubiquitin ligases. This protein belongs to translation initiation factor 3 (eIF3) superfamily. It is involved in the regulation of cell cycle and likely to be a cellular cofactor for HIV-1 accessory gene product Vpr. [provided by RefSeq, Jul 2008]
UniProt Code:	Q7L5N1
NCBI GenInfo Identifier:	55976470
NCBI Gene ID:	10980
NCBI Accession:	Q7L5N1.1
UniProt Secondary Accession:	Q7L5N1,O15387, A4D2A3,
UniProt Related Accession:	Q7L5N1
Molecular Weight:	327
NCBI Full Name:	COP9 signalosome complex subunit 6
NCBI Synonym Full Names:	COP9 signalosome subunit 6
NCBI Official Symbol:	COPS6
NCBI Official Synonym Symbols:	CSN6; MOV34-34KD
NCBI Protein Information:	COP9 signalosome complex subunit 6; SGN6; hVIP; H_NH0506M12.12; MOV34 homolog, 34 kD; signalosome subunit 6; vpr-interacting protein; COP9 subunit 6 (MOV34 homolog, 34 kD); JAB1-containing signalosome subunit 6; COP9 constitutive photomorphogenic homolog subunit 6
UniProt Protein Name:	COP9 signalosome complex subunit 6
UniProt Synonym Protein Names:	JAB1-containing signalosome subunit 6; MOV34 homolog; Vpr-interacting protein; hVIP
UniProt Gene Name:	COPS6
UniProt Entry Name:	CSN6_HUMAN

	Western blot analysis of extracts of various cell lines, using COPS6 antibody (CAB7072) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunofluorescence analysis of MCF7 cells using COPS6 antibody (CAB7072). Blue: DAPI for nuclear staining.