Cell Biology Antibodies 10

Anti-COPS3 Antibody (CAB7017)

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SKU:
CAB7017
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-COPS3 Antibody
Antibody SKU:CAB7017
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 194-423 of human COPS3 (NP_003644.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:SW480, HeLa, 293T, Mouse heart, Rat heart, Rat brian
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 194-423 of human COPS3 (NP_003644.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GLKN FERA LYFY EQAI TTPA MAVS HIML ESYK KYIL VSLI LLGK VQQL PKYT SQIV GRFI KPLS NAYH ELAQ VYST NNPS ELRN LVNK HSET FTRD NNMG LVKQ CLSS LYKK NIQR LTKT FLTL SLQD MASR VQLS GPQE AEKY VLHM IEDG EIFA SINQ KDGM VSFH DNPE KYNN PAML HNID QEML KCIE LDER LKAM DQEI TVNP QFVQ KSMG SQED DSGN KPSS YS
Gene ID:8533
Uniprot:Q9UNS2
Cellular Location:Cytoplasm, Nucleus
Calculated MW:45kDa/47kDa
Observed MW:48kDa
Synonyms:COPS3, CSN3, SGN3
Background:The protein encoded by this gene possesses kinase activity that phosphorylates regulators involved in signal transduction. It phosphorylates I kappa-Balpha, p105, and c-Jun. It acts as a docking site for complex-mediated phosphorylation. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Several transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:COPS3: Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Belongs to the CSN3 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: nucleoplasm; signalosome; cytoplasm; nucleus

Molecular Function:protein binding

Biological Process: ubiquitin-dependent protein catabolic process; response to light stimulus; cullin deneddylation; in utero embryonic development; signal transduction

NCBI Summary:The protein encoded by this gene possesses kinase activity that phosphorylates regulators involved in signal transduction. It phosphorylates I kappa-Balpha, p105, and c-Jun. It acts as a docking site for complex-mediated phosphorylation. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]
UniProt Code:Q9UNS2
NCBI GenInfo Identifier:55976621
NCBI Gene ID:8533
NCBI Accession:Q9UNS2.3
UniProt Secondary Accession:Q9UNS2,O43191, Q7LDR6, B2R683, B4DY81,
UniProt Related Accession:Q9UNS2
Molecular Weight:423
NCBI Full Name:COP9 signalosome complex subunit 3
NCBI Synonym Full Names:COP9 signalosome subunit 3
NCBI Official Symbol:COPS3  
NCBI Official Synonym Symbols:CSN3; SGN3  
NCBI Protein Information:COP9 signalosome complex subunit 3; signalosome subunit 3; COP9 complex subunit 3; JAB1-containing signalosome subunit 3; COP9 constitutive photomorphogenic homolog subunit 3
UniProt Protein Name:COP9 signalosome complex subunit 3
UniProt Synonym Protein Names:JAB1-containing signalosome subunit 3
UniProt Gene Name:COPS3  
UniProt Entry Name:CSN3_HUMAN
Anti-COPS3 Antibody (CAB7017)Western blot analysis of extracts of various cell lines, using COPS3 antibody (CAB7017) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-COPS3 Antibody (CAB7017)Immunofluorescence analysis of MCF7 cells using COPS3 antibody (CAB7017). Blue: DAPI for nuclear staining.
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