Cell Biology Antibodies 10

Anti-COPB2 Antibody (CAB7036)

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SKU:
CAB7036
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-COPB2 Antibody
Antibody SKU:CAB7036
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 657-906 of human COPB2 (NP_004757.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:SW480, MCF7, HeLa, HepG2, Jurkat, Mouse liver, Mouse kidney, Mouse stomach
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 657-906 of human COPB2 (NP_004757.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:AYQL AVEA ESEQ KWKQ LAEL AISK CQFG LAQE CLHH AQDY GGLL LLAT ASGN ANMV NKLA EGAE RDGK NNVA FMSY FLQG KVDA CLEL LIRT GRLP EAAF LART YLPS QVSR VVKL WREN LSKV NQKA AESL ADPT EYEN LFPG LKEA FVVE EWVK ETHA DLWP AKQY PLVT PNEE RNVM EEGK DFQP SRST AQQE LDGK PASP TPVI VASH TANK EEKS LLEL EVDL DNLE LEDI DTTD INLD EDIL DD
Gene ID:9276
Uniprot:P35606
Cellular Location:COPI-coated vesicle membrane, Cytoplasm, Cytoplasmic side, Cytoplasmic vesicle, Golgi apparatus membrane, Peripheral membrane protein, cytosol
Calculated MW:99kDa/102kDa
Observed MW:102kDa
Synonyms:COPB2, beta-COP
Background:The Golgi coatomer complex (see MIM 601924) constitutes the coat of nonclathrin-coated vesicles and is essential for Golgi budding and vesicular trafficking. It consists of 7 protein subunits, including COPB2.[supplied by OMIM, Jul 2002]
UniProt Protein Function:COPB2: The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Belongs to the WD repeat COPB2 family.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Vesicle

Chromosomal Location of Human Ortholog: 3q23

Cellular Component: COPI vesicle coat; cytosol; endoplasmic reticulum membrane; Golgi membrane; transport vesicle

Molecular Function:protein binding

Biological Process: ER to Golgi vesicle-mediated transport; intra-Golgi vesicle-mediated transport; intracellular protein transport; retrograde vesicle-mediated transport, Golgi to ER

NCBI Summary:The Golgi coatomer complex (see MIM 601924) constitutes the coat of nonclathrin-coated vesicles and is essential for Golgi budding and vesicular trafficking. It consists of 7 protein subunits, including COPB2.[supplied by OMIM, Jul 2002]
UniProt Code:P35606
NCBI GenInfo Identifier:544076
NCBI Gene ID:9276
NCBI Accession:P35606.2
UniProt Secondary Accession:P35606,B4DZI8,
UniProt Related Accession:P35606
Molecular Weight:99,046 Da
NCBI Full Name:Coatomer subunit beta'
NCBI Synonym Full Names:coatomer protein complex subunit beta 2
NCBI Official Symbol:COPB2  
NCBI Official Synonym Symbols:beta'-COP  
NCBI Protein Information:coatomer subunit beta'
UniProt Protein Name:Coatomer subunit beta'
UniProt Synonym Protein Names:Beta'-coat protein; Beta'-COP; p102
UniProt Gene Name:COPB2  
UniProt Entry Name:COPB2_HUMAN
Anti-COPB2 Antibody (CAB7036)Western blot analysis of extracts of various cell lines, using COPB2 antibody (CAB7036) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-COPB2 Antibody (CAB7036)Immunohistochemistry of paraffin-embedded rat brain using COPB2 antibody (CAB7036) at dilution of 1:100 (40x lens).
Anti-COPB2 Antibody (CAB7036)Immunohistochemistry of paraffin-embedded mouse brain using COPB2 antibody (CAB7036) at dilution of 1:100 (40x lens).
Anti-COPB2 Antibody (CAB7036)Immunofluorescence analysis of HeLa cells using COPB2 antibody (CAB7036) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-COPB2 Antibody (CAB7036)Immunofluorescence analysis of NIH/3T3 cells using COPB2 antibody (CAB7036) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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